Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1044638..1045197 Replicon chromosome
Accession NZ_LT962936
Organism Brucella melitensis isolate

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag C0M12_RS05345 Protein ID WP_002966807.1
Coordinates 1044638..1044883 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag C0M12_RS05350 Protein ID WP_004683740.1
Coordinates 1044883..1045197 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0M12_RS05325 1040484..1041935 - 1452 WP_006135932.1 hypothetical protein -
C0M12_RS05330 1042347..1043039 - 693 WP_006135935.1 hypothetical protein -
C0M12_RS05340 1043867..1044505 - 639 Protein_981 tRNA-guanine transglycosylase -
C0M12_RS05345 1044638..1044883 + 246 WP_002966807.1 BrnT family toxin Toxin
C0M12_RS05350 1044883..1045197 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
C0M12_RS05360 1045545..1045715 + 171 WP_002964095.1 DUF1508 domain-containing protein -
C0M12_RS05365 1046063..1046773 - 711 WP_004683739.1 porin family protein -
C0M12_RS05370 1047119..1047595 - 477 WP_002966806.1 hypothetical protein -
C0M12_RS05375 1047618..1047893 - 276 WP_002964092.1 hypothetical protein -
C0M12_RS05380 1047890..1048120 - 231 WP_002964091.1 hypothetical protein -
C0M12_RS05385 1048117..1048821 - 705 WP_005968944.1 hypothetical protein -
C0M12_RS05390 1048871..1049074 + 204 WP_002964088.1 hypothetical protein -
C0M12_RS05395 1049071..1049286 + 216 WP_005968947.1 hypothetical protein -
C0M12_RS05400 1049289..1049492 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA 1038926..1051264 12338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T293881 WP_002966807.1 NZ_LT962936:1044638-1044883 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT293881 WP_004683740.1 NZ_LT962936:1044883-1045197 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References