293880

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1044688..1045247 Replicon chromosome
Accession NZ_LT962934
Organism Brucella melitensis isolate

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag C0M13_RS05355 Protein ID WP_002966807.1
Coordinates 1044688..1044933 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag C0M13_RS05360 Protein ID WP_004683740.1
Coordinates 1044933..1045247 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0M13_RS05335 1040534..1041985 - 1452 WP_006135932.1 hypothetical protein -
C0M13_RS05340 1042397..1043089 - 693 WP_006135935.1 hypothetical protein -
C0M13_RS05350 1043917..1044555 - 639 Protein_981 tRNA-guanine transglycosylase -
C0M13_RS05355 1044688..1044933 + 246 WP_002966807.1 BrnT family toxin Toxin
C0M13_RS05360 1044933..1045247 + 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
C0M13_RS05365 1045363..1045596 - 234 WP_002969999.1 hypothetical protein -
C0M13_RS05370 1045595..1045765 + 171 WP_002964095.1 DUF1508 domain-containing protein -
C0M13_RS05375 1046113..1046823 - 711 WP_004683739.1 porin family protein -
C0M13_RS05380 1047169..1047645 - 477 WP_002966806.1 hypothetical protein -
C0M13_RS05385 1047668..1047943 - 276 WP_002964092.1 hypothetical protein -
C0M13_RS05390 1047940..1048170 - 231 WP_002964091.1 hypothetical protein -
C0M13_RS05395 1048167..1048871 - 705 WP_005968944.1 hypothetical protein -
C0M13_RS05400 1048921..1049124 + 204 WP_002964088.1 hypothetical protein -
C0M13_RS05405 1049121..1049336 + 216 WP_005968947.1 hypothetical protein -
C0M13_RS05410 1049339..1049542 + 204 WP_004683737.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbdA / wboA 1037420..1051314 13894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T293880 WP_002966807.1 NZ_LT962934:1044688-1044933 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T293880 NZ_LT962934:1044688-1044933 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT293880 WP_004683740.1 NZ_LT962934:1044933-1045247 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT293880 NZ_LT962934:1044933-1045247 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References