Detailed information of TA system TA029380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4106580..4107188
• Replicon: chromosome
• Accession: NC_021149
• Organism: Azotobacter vinelandii CA

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: M9YR94
• Locus tag: AVCA_RS18480
• Protein ID: WP_012702568.1
• Coordinates: 4106898..4107188 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AVCA_RS18475
• Protein ID: WP_012702567.1
• Coordinates: 4106580..4106888 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA_RS26160	4101761..4102099	+	339	WP_144061665.1	hypothetical protein	-
AVCA_RS26165	4102105..4102323	+	219	WP_144061666.1	hypothetical protein	-
AVCA_RS18435	4102351..4103241	-	891	Protein_3702	IS3-like element ISAzvi9 family transposase	-
AVCA_RS18445	4103315..4104180	+	866	Protein_3703	IS110 family transposase	-
AVCA_RS18450	4104434..4104697	-	264	Protein_3704	transposase	-
AVCA_RS18455	4104908..4105132	+	225	WP_012702563.1	AlpA family phage regulatory protein	-
AVCA_RS18460	4105134..4105517	+	384	WP_012702564.1	hypothetical protein	-
AVCA_RS18465	4105514..4106185	+	672	WP_012702565.1	Rha family transcriptional regulator	-
AVCA_RS18470	4106182..4106430	+	249	WP_012702566.1	hypothetical protein	-
AVCA_RS18475	4106580..4106888	-	309	WP_012702567.1	putative addiction module antidote protein	Antitoxin
AVCA_RS18480	4106898..4107188	-	291	WP_012702568.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AVCA_RS26525	4107543..4107716	+	174	WP_012702569.1	hypothetical protein	-
AVCA_RS18485	4107713..4107967	+	255	WP_012702570.1	hypothetical protein	-
AVCA_RS18490	4107964..4108263	+	300	WP_012702571.1	hypothetical protein	-
AVCA_RS26170	4108311..4108676	+	366	WP_012702573.1	hypothetical protein	-
AVCA_RS18500	4108755..4109141	+	387	WP_012702575.1	toprim domain-containing protein	-
AVCA_RS18505	4109138..4109530	+	393	WP_012702576.1	hypothetical protein	-
AVCA_RS18510	4110234..4110437	+	204	WP_012702577.1	hypothetical protein	-
AVCA_RS18515	4110932..4111141	+	210	WP_012702578.1	hypothetical protein	-
AVCA_RS18520	4111257..4111493	-	237	WP_012702579.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4100549..4116064	15515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10796.24 Da
• Isoelectric Point: 8.0567

>T29380 WP_012702568.1 NC_021149:c4107188-4106898 [Azotobacter vinelandii CA]
MKSILTTDVFDRWFSGLRDRRAAVRVQARIDRAEDGNFGDCEPVGEGVSEMRIHYGPGYRVYFTQRGHELVILLAGGDKS
SQAKDIKAAIDMARHL

Nucleotide

• Length: 291 bp

>T29380 NC_021149:c4107188-4106898 [Azotobacter vinelandii CA]
ATGAAAAGCATCCTCACCACGGACGTGTTCGACCGCTGGTTCTCCGGCCTGCGGGACAGGCGGGCGGCAGTGCGAGTACA
GGCCCGCATTGACCGTGCCGAAGATGGCAACTTCGGCGATTGCGAGCCGGTCGGCGAAGGCGTGTCGGAGATGCGCATCC
ACTACGGGCCGGGCTATCGCGTGTATTTCACGCAACGCGGGCACGAGTTGGTCATCCTGCTGGCCGGTGGCGACAAGTCC
AGCCAAGCGAAAGACATCAAGGCCGCTATCGACATGGCGCGGCATCTGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10922.51 Da
• Isoelectric Point: 6.4795

>AT29380 WP_012702567.1 NC_021149:c4106888-4106580 [Azotobacter vinelandii CA]
MTTVKLRKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVIQALGLRLHVEAGAAHTH

Nucleotide

• Length: 309 bp

>AT29380 NC_021149:c4106888-4106580 [Azotobacter vinelandii CA]
ATGACTACCGTCAAACTGCGCAAGTGGGATAGCGCCGAACACCTCAAGACCGATGAGGACATGGCGCTTTATCTTGAGGC
TTGCATCGAGGAGGCTGGCGACGATGCGGCGTTCATCGCCAAGGCTCTCGGCACCATCGCCCGCGCCAAGGGCATGGCCC
AATTGGCACGAGACACCGGCCTCGGGCGCGAAAGTCTGTACAAGGCGCTGTCCGGCGAGGGCAACCCCAGCTTCGCCACC
ATCCTCAAGGTGATACAGGCGCTCGGCCTCCGGCTTCACGTTGAAGCCGGTGCGGCGCACACTCACTGA

Structures

Toxin

• AlphaFold DB: M9YR94