Detailed information of TA system TA029377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3703870..3704399
• Replicon: chromosome
• Accession: NC_021149
• Organism: Azotobacter vinelandii CA

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M9YLU7
• Locus tag: AVCA_RS16550
• Protein ID: WP_012702163.1
• Coordinates: 3703870..3704193 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AVCA_RS16555
• Protein ID: WP_041807346.1
• Coordinates: 3704190..3704399 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA_RS16525	3699070..3700002	+	933	WP_012702159.1	TIGR03756 family integrating conjugative element protein	-
AVCA_RS16530	3700025..3701389	+	1365	WP_012702160.1	integrating conjugative element protein	-
AVCA_RS16535	3701396..3701752	+	357	WP_012702161.1	hypothetical protein	-
AVCA_RS16540	3701749..3703314	+	1566	WP_012702162.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
AVCA_RS16545	3703332..3703637	-	306	WP_041807344.1	hypothetical protein	-
AVCA_RS25300	3703690..3703863	-	174	WP_081434905.1	hypothetical protein	-
AVCA_RS16550	3703870..3704193	-	324	WP_012702163.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AVCA_RS16555	3704190..3704399	-	210	WP_041807346.1	antitoxin MazE family protein	Antitoxin
AVCA_RS16560	3704593..3705195	+	603	WP_012702164.1	DUF4852 domain-containing protein	-
AVCA_RS16565	3705308..3705547	-	240	WP_175556057.1	Arc family DNA-binding protein	-
AVCA_RS16570	3705897..3706930	+	1034	Protein_3320	IS630 family transposase	-
AVCA_RS25310	3707347..3707985	+	639	WP_012702167.1	hypothetical protein	-
AVCA_RS16575	3708207..3708914	-	708	WP_012702168.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3617335..3712033	94698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (5-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11433.43 Da
• Isoelectric Point: 9.7779

>T29377 WP_012702163.1 NC_021149:c3704193-3703870 [Azotobacter vinelandii CA]
MNRGAVVVVALQGAYGKPRPALVIQSDLFSALPSVTILPITSDLREAPLYRIDIEAAPRTGLHKPSQIMVDKPQTIPREK
VGEVIGSVNAETMLAVSRSLTVFLGIA

Nucleotide

• Length: 324 bp

>T29377 NC_021149:c3704193-3703870 [Azotobacter vinelandii CA]
ATGAATCGAGGTGCCGTGGTGGTGGTCGCCTTGCAGGGCGCCTACGGAAAGCCTCGCCCGGCGTTGGTCATCCAGTCCGA
TCTGTTCTCGGCGTTGCCTTCCGTGACCATTCTTCCTATCACGAGCGATTTGCGGGAGGCGCCGCTTTATCGCATCGACA
TCGAGGCCGCGCCGAGAACCGGGTTGCACAAGCCATCACAGATCATGGTGGACAAGCCGCAAACGATCCCCCGCGAGAAA
GTGGGCGAGGTGATCGGCTCGGTCAACGCAGAAACCATGCTGGCCGTGAGTCGGTCGCTAACCGTGTTTCTCGGTATTGC
TTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8061.07 Da
• Isoelectric Point: 5.1502

>AT29377 WP_041807346.1 NC_021149:c3704399-3704190 [Azotobacter vinelandii CA]
MQTVRERVRKRREALRAAGLRPVQIWVPDTRAPGFAEEARRQCLLVQNDPQEKESLEWLESAADTEGWE

Nucleotide

• Length: 210 bp

>AT29377 NC_021149:c3704399-3704190 [Azotobacter vinelandii CA]
ATGCAGACCGTGCGTGAGCGGGTCAGGAAGCGGCGCGAGGCGCTGCGGGCTGCTGGGTTGCGCCCGGTGCAAATCTGGGT
GCCGGACACCCGTGCTCCCGGCTTTGCGGAAGAGGCGAGGCGGCAGTGCCTTCTCGTACAGAATGATCCGCAAGAGAAAG
AAAGTCTGGAGTGGCTGGAAAGCGCGGCTGACACAGAGGGGTGGGAATGA

Structures

Toxin

• AlphaFold DB: M9YLU7