Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4156290..4156787 Replicon chromosome
Accession NZ_LT906474
Organism Escherichia coli strain NCTC122

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CKV98_RS21155 Protein ID WP_001173343.1
Coordinates 4156290..4156463 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CKV98_RS21160 Protein ID WP_000398619.1
Coordinates 4156491..4156787 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKV98_RS21130 4151620..4152195 + 576 WP_001188520.1 transcriptional regulator -
CKV98_RS21145 4153863..4154417 + 555 Protein_3886 dipeptide/tripeptide permease DtpC -
CKV98_RS21150 4154654..4156171 + 1518 WP_001295074.1 lysine--tRNA ligase -
CKV98_RS21155 4156290..4156463 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CKV98_RS21160 4156491..4156787 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CKV98_RS21170 4157014..4157286 - 273 WP_000405647.1 N-acetyltransferase -
CKV98_RS21175 4157298..4157528 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CKV98_RS21180 4157709..4159340 + 1632 WP_047402662.1 sensor histidine kinase -
CKV98_RS21185 4159337..4160056 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T293761 WP_001173343.1 NZ_LT906474:c4156463-4156290 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT293761 WP_000398619.1 NZ_LT906474:c4156787-4156491 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References