Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1688284..1688932 Replicon chromosome
Accession NC_021082
Organism Mannheimia haemolytica M42548

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag MHH_RS22580 Protein ID WP_006251221.1
Coordinates 1688756..1688932 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag MHH_RS22575 Protein ID WP_006251222.1
Coordinates 1688284..1688700 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MHH_RS22535 1684532..1684966 + 435 WP_006251230.1 hypothetical protein -
MHH_RS22540 1684981..1685970 + 990 WP_006251229.1 encapsulin -
MHH_RS28495 1685982..1686287 + 306 WP_015587049.1 hypothetical protein -
MHH_RS22550 1686290..1686667 + 378 WP_006251227.1 hypothetical protein -
MHH_RS22555 1686667..1687011 + 345 WP_006251226.1 hypothetical protein -
MHH_RS22560 1687013..1687381 + 369 WP_006251225.1 hypothetical protein -
MHH_RS22565 1687378..1687758 + 381 WP_006251224.1 hypothetical protein -
MHH_RS22570 1687762..1688244 + 483 WP_006251223.1 phage tail protein -
MHH_RS22575 1688284..1688700 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MHH_RS22580 1688756..1688932 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
MHH_RS22585 1689028..1689699 + 672 WP_006251220.1 hypothetical protein -
MHH_RS22590 1689714..1689947 - 234 WP_020824316.1 hypothetical protein -
MHH_RS22595 1690116..1690574 + 459 WP_006251218.1 hypothetical protein -
MHH_RS22605 1691119..1693614 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1582383..1709065 126682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T29369 WP_006251221.1 NC_021082:c1688932-1688756 [Mannheimia haemolytica M42548]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T29369 NC_021082:c1688932-1688756 [Mannheimia haemolytica M42548]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT29369 WP_006251222.1 NC_021082:c1688700-1688284 [Mannheimia haemolytica M42548]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT29369 NC_021082:c1688700-1688284 [Mannheimia haemolytica M42548]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References