Detailed information of TA system TA293688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2858016..2859490
• Replicon: chromosome
• Accession: NZ_LT906442
• Organism: Legionella waltersii strain NCTC13017

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W1AGC7
• Locus tag: CKW04_RS13200
• Protein ID: WP_028378775.1
• Coordinates: 2858016..2858885 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: CKW04_RS13205
• Protein ID: WP_028378776.1
• Coordinates: 2858882..2859490 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKW04_RS17375	2853787..2853948	+	162	WP_157066268.1	hypothetical protein	-
CKW04_RS13175	2854124..2855722	-	1599	WP_080274386.1	relaxase/mobilization nuclease domain-containing protein	-
CKW04_RS13180	2855719..2856072	-	354	WP_028378771.1	conjugal transfer transcriptional regulator TraJ	-
CKW04_RS13185	2856468..2856812	+	345	WP_028378772.1	TraK family protein	-
CKW04_RS13190	2856812..2857537	+	726	WP_028378773.1	conjugal transfer protein TraL	-
CKW04_RS13195	2857537..2857974	+	438	WP_028378774.1	conjugal transfer protein TraM	-
CKW04_RS13200	2858016..2858885	-	870	WP_028378775.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CKW04_RS13205	2858882..2859490	-	609	WP_028378776.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CKW04_RS13210	2860833..2862347	+	1515	WP_058479876.1	Fic family protein	-
CKW04_RS13215	2862627..2863079	-	453	WP_058479951.1	hypothetical protein	-
CKW04_RS13220	2863133..2864104	-	972	WP_058479875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sidB / lem8 / lpg2370	2766469..2900080	133611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-260)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (10-56)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32560.44 Da
• Isoelectric Point: 6.9833

>T293688 WP_028378775.1 NZ_LT906442:c2858885-2858016 [Legionella waltersii]
MTKNIGESVRSRLKNIAVKEGSDFNAVLTRYGLERLLYRIGESEYSKQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICRISADDGISFLSESVTADTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLS
DLPAPKIRTYPIYTVIAEKLHAIALLGMTNSRLKDYLDLYVLLGNEQIDNQILAKAIHATFNRRSMDLPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKSLTEVIAVIRNLIQVPYSLSTHPNFSR

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22968.85 Da
• Isoelectric Point: 9.0310

>AT293688 WP_028378776.1 NZ_LT906442:c2859490-2858882 [Legionella waltersii]
MKTEATYKQKVLQMASKSGIVRMSDLTERGITRATISRLVSENKLEKLASGLYCLPETEFSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYNFAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

Structures

Toxin

• AlphaFold DB: A0A0W1AGC7