Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 528639..529275 Replicon chromosome
Accession NZ_LT906438
Organism Bacillus pumilus strain NCTC10337

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag CKW02_RS02580 Protein ID WP_003214169.1
Coordinates 528925..529275 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag CKW02_RS02575 Protein ID WP_003214273.1
Coordinates 528639..528920 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKW02_RS02555 524786..525391 - 606 WP_003214209.1 rhomboid family intramembrane serine protease -
CKW02_RS02560 525486..525851 + 366 WP_003214420.1 holo-ACP synthase -
CKW02_RS02565 526012..527028 + 1017 WP_095117747.1 outer membrane lipoprotein carrier protein LolA -
CKW02_RS02570 527164..528345 + 1182 WP_003214072.1 alanine racemase -
CKW02_RS02575 528639..528920 + 282 WP_003214273.1 hypothetical protein Antitoxin
CKW02_RS02580 528925..529275 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CKW02_RS02585 529390..530220 + 831 WP_003214372.1 STAS domain-containing protein -
CKW02_RS02590 530225..530593 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
CKW02_RS02595 530596..530997 + 402 WP_003214085.1 anti-sigma regulatory factor -
CKW02_RS02600 531008..532015 + 1008 WP_003213895.1 PP2C family protein-serine/threonine phosphatase -
CKW02_RS02605 532075..532404 + 330 WP_003213847.1 anti-sigma factor antagonist -
CKW02_RS02610 532401..532889 + 489 WP_003214356.1 anti-sigma B factor RsbW -
CKW02_RS02615 532855..533643 + 789 WP_003214304.1 RNA polymerase sigma factor SigB -
CKW02_RS02620 533643..534242 + 600 WP_003214034.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T293683 WP_003214169.1 NZ_LT906438:528925-529275 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT293683 WP_003214273.1 NZ_LT906438:528639-528920 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References