Detailed information of TA system TA029368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1639932..1640572
• Replicon: chromosome
• Accession: NC_021082
• Organism: Mannheimia haemolytica M42548

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: MHH_RS22210
• Protein ID: WP_006250075.1
• Coordinates: 1640390..1640572 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: MHH_RS22205
• Protein ID: WP_006250074.1
• Coordinates: 1639932..1640351 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHH_RS22185	1635479..1636285	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
MHH_RS22190	1636335..1637741	+	1407	WP_006253513.1	YdgA family protein	-
MHH_RS22195	1637808..1638814	-	1007	Protein_1609	IS481 family transposase	-
MHH_RS22200	1638952..1639596	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
MHH_RS22205	1639932..1640351	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MHH_RS22210	1640390..1640572	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
MHH_RS22215	1640704..1641189	-	486	WP_015484250.1	hypothetical protein	-
MHH_RS22220	1641194..1641574	-	381	WP_006248823.1	hypothetical protein	-
MHH_RS22225	1641649..1642332	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
MHH_RS22230	1642463..1642663	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
MHH_RS28620	1642684..1643751	+	1068	WP_006253284.1	hypothetical protein	-
MHH_RS28480	1643761..1643919	+	159	WP_006247809.1	hypothetical protein	-
MHH_RS22240	1643979..1645019	-	1041	WP_015587043.1	IS481 family transposase	-
MHH_RS22245	1645136..1645435	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1582383..1709065	126682
	-	flank	IS/Tn	-	-	1643979..1645019	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T29368 WP_006250075.1 NC_021082:c1640572-1640390 [Mannheimia haemolytica M42548]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T29368 NC_021082:c1640572-1640390 [Mannheimia haemolytica M42548]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT29368 WP_006250074.1 NC_021082:c1640351-1639932 [Mannheimia haemolytica M42548]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT29368 NC_021082:c1640351-1639932 [Mannheimia haemolytica M42548]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9