293654

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3659551..3661271 Replicon chromosome
Accession NZ_LT903847
Organism Escherichia coli O127:H6 isolate EPEC1

Toxin (Protein)


Gene name pmenT Uniprot ID B7UP20
Locus tag BQ9550_RS19450 Protein ID WP_000312835.1
Coordinates 3659551..3660207 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag BQ9550_RS19455 Protein ID WP_000634203.1
Coordinates 3660204..3661271 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9550_RS19435 3655461..3655700 + 240 WP_000343040.1 hypothetical protein -
BQ9550_RS19440 3655740..3658178 + 2439 WP_000985463.1 type I restriction-modification system subunit M -
BQ9550_RS19445 3658190..3659539 + 1350 WP_001386438.1 restriction endonuclease subunit S -
BQ9550_RS19450 3659551..3660207 + 657 WP_000312835.1 DUF4433 domain-containing protein Toxin
BQ9550_RS19455 3660204..3661271 + 1068 WP_000634203.1 macro domain-containing protein Antitoxin
BQ9550_RS19460 3661290..3664385 + 3096 WP_000108733.1 HsdR family type I site-specific deoxyribonuclease -
BQ9550_RS19465 3664385..3665101 + 717 WP_001122107.1 M48 family metallopeptidase -
BQ9550_RS19470 3665271..3665471 + 201 Protein_3572 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3637799..3693010 55211
- inside Prophage - - 3637799..3699419 61620


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25276.63 Da        Isoelectric Point: 6.5796

>T293654 WP_000312835.1 NZ_LT903847:3659551-3660207 [Escherichia coli O127:H6]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKRRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRSGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT293654 WP_000634203.1 NZ_LT903847:3660204-3661271 [Escherichia coli O127:H6]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376HLR4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References