Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4213750..4214297 Replicon chromosome
Accession NC_021066
Organism Raoultella ornithinolytica B6

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag RORB6_RS25650 Protein ID WP_048025274.1
Coordinates 4213750..4214058 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag RORB6_RS19195 Protein ID WP_004869059.1
Coordinates 4214061..4214297 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RORB6_RS19165 4208938..4209678 + 741 WP_015585491.1 4-oxalmesaconate hydratase -
RORB6_RS19170 4209675..4210388 + 714 WP_015585492.1 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase -
RORB6_RS19175 4210393..4211484 + 1092 WP_015585493.1 4-oxalomesaconate tautomerase -
RORB6_RS19180 4211481..4212347 + 867 WP_015585494.1 NAD(P)-dependent oxidoreductase -
RORB6_RS19185 4212348..4212623 - 276 WP_167324217.1 hypothetical protein -
RORB6_RS19190 4212732..4213634 - 903 WP_015585496.1 EamA family transporter -
RORB6_RS25650 4213750..4214058 - 309 WP_048025274.1 CcdB family protein Toxin
RORB6_RS19195 4214061..4214297 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
RORB6_RS19200 4214403..4215836 - 1434 WP_004869058.1 PTS N-acetylmuramic acid transporter subunit IIBC -
RORB6_RS19205 4215861..4216763 - 903 WP_015585498.1 N-acetylmuramic acid 6-phosphate etherase -
RORB6_RS19210 4216928..4218091 - 1164 WP_004869055.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T29361 WP_048025274.1 NC_021066:c4214058-4213750 [Raoultella ornithinolytica B6]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T29361 NC_021066:c4214058-4213750 [Raoultella ornithinolytica B6]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCGG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT29361 WP_004869059.1 NC_021066:c4214297-4214061 [Raoultella ornithinolytica B6]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT29361 NC_021066:c4214297-4214061 [Raoultella ornithinolytica B6]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References