Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4257109..4257606 Replicon chromosome
Accession NZ_LT883142
Organism Escherichia coli isolate 6666666.257727.embl

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC1094V2_RS23550 Protein ID WP_001173343.1
Coordinates 4257109..4257282 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC1094V2_RS23555 Protein ID WP_000398619.1
Coordinates 4257310..4257606 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1094V2_RS23540 4253774..4255231 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
EC1094V2_RS23545 4255468..4256985 + 1518 WP_096321255.1 lysine--tRNA ligase -
EC1094V2_RS23550 4257109..4257282 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC1094V2_RS23555 4257310..4257606 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC1094V2_RS23565 4257833..4258105 - 273 WP_000405647.1 N-acetyltransferase -
EC1094V2_RS23570 4258117..4258347 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
EC1094V2_RS23575 4258528..4260159 + 1632 WP_001216477.1 sensor histidine kinase -
EC1094V2_RS23580 4260156..4260875 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T293605 WP_001173343.1 NZ_LT883142:c4257282-4257109 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT293605 WP_000398619.1 NZ_LT883142:c4257606-4257310 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References