Detailed information of TA system TA293580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 5512119..5512998
• Replicon: chromosome
• Accession: NZ_LT855380
• Organism: Pseudomonas viridiflava strain CFBP 1590 isolate E12-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5E6PR12
• Locus tag: CFBP1590_RS24720
• Protein ID: WP_063625668.1
• Coordinates: 5512645..5512998 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5E6PJ22
• Locus tag: CFBP1590_RS24715
• Protein ID: WP_029243148.1
• Coordinates: 5512119..5512592 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBP1590_RS24700	5508536..5509393	+	858	WP_029243146.1	poly(3-hydroxyalkanoate) depolymerase	-
CFBP1590_RS24705	5509558..5511243	+	1686	WP_088236102.1	class II poly(R)-hydroxyalkanoic acid synthase	-
CFBP1590_RS24710	5511324..5511938	+	615	WP_004882680.1	TetR/AcrR family transcriptional regulator	-
CFBP1590_RS24715	5512119..5512592	-	474	WP_029243148.1	transcriptional regulator	Antitoxin
CFBP1590_RS24720	5512645..5512998	-	354	WP_063625668.1	type II toxin-antitoxin system HigB family toxin	Toxin
CFBP1590_RS24725	5513177..5513962	-	786	WP_088236103.1	phasin family protein	-
CFBP1590_RS24730	5513973..5514446	-	474	WP_088236104.1	phasin family protein	-
CFBP1590_RS24735	5514607..5514885	-	279	WP_004882685.1	polyhydroxyalkanoic acid system family protein	-
CFBP1590_RS24740	5515030..5515800	+	771	WP_004882686.1	bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE	-
CFBP1590_RS24745	5515800..5516423	+	624	WP_025993915.1	SCP2 sterol-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13526.70 Da
• Isoelectric Point: 10.0467

>T293580 WP_063625668.1 NZ_LT855380:c5512998-5512645 [Pseudomonas viridiflava]
MRVIAKTTVAKAIEAHGQWKAALSLWLTTFDRATLRFESFEQLRDTWVTASGWNIDRIPCSKLRQPSVKGPLDVYVFDIK
KNECRIIAWINTRNGTIYIKDILPHAGYDKWCKSDVK

Nucleotide

• Length: 354 bp

>T293580 NZ_LT855380:c5512998-5512645 [Pseudomonas viridiflava]
ATGCGGGTAATAGCAAAAACGACGGTTGCGAAAGCCATCGAAGCTCATGGTCAGTGGAAGGCGGCACTGAGTTTGTGGCT
GACCACCTTTGACAGGGCGACTCTGCGCTTTGAGTCCTTCGAGCAGCTTCGAGATACCTGGGTCACCGCTTCTGGATGGA
ACATTGACCGAATTCCCTGCTCGAAGCTGAGACAGCCGAGCGTTAAAGGGCCTCTTGATGTGTATGTCTTCGACATCAAG
AAAAATGAGTGCCGGATTATTGCGTGGATTAATACCCGGAATGGCACTATTTATATAAAGGATATTCTGCCCCACGCCGG
TTATGACAAGTGGTGCAAGAGTGACGTTAAGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17239.59 Da
• Isoelectric Point: 4.8200

>AT293580 WP_029243148.1 NZ_LT855380:c5512592-5512119 [Pseudomonas viridiflava]
MKHDKNGATGLDSFIANISPMLDGLSDQIATMAQLLAACHDPVKSDTDLKGRMALIDELYSHADSEDHAAARFAELVAER
VYEYETETVLIPYSSQAEALAFLISDRGVRQKDLSGVATQSAVSEILNNKRKMTVAQIKGFAEFFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT293580 NZ_LT855380:c5512592-5512119 [Pseudomonas viridiflava]
ATGAAGCATGATAAGAACGGAGCCACTGGGCTGGATTCCTTCATCGCTAACATTTCACCGATGCTTGATGGCTTAAGCGA
TCAGATAGCAACGATGGCCCAGTTATTGGCTGCCTGTCATGATCCCGTCAAGAGTGACACTGACTTGAAGGGGCGGATGG
CGCTGATCGATGAGCTTTACTCTCATGCAGACAGTGAAGATCACGCAGCCGCGCGCTTTGCGGAGCTGGTGGCCGAGCGT
GTGTATGAGTACGAGACTGAAACCGTTTTGATTCCTTACTCATCTCAGGCGGAAGCACTGGCTTTCCTGATTTCGGACAG
GGGAGTCAGACAGAAGGATTTGTCAGGTGTTGCCACTCAAAGTGCTGTTTCCGAGATACTGAACAACAAGAGGAAAATGA
CGGTTGCTCAGATAAAAGGCTTTGCAGAATTCTTTAAAGTGCCCGTCGAGTTTTTCATGCATGGCGTCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A5E6PR12

Antitoxin

• AlphaFold DB: A0A5E6PJ22