Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1100..1692 Replicon plasmid pWI2-mcr
Accession NZ_LT838201
Organism Escherichia coli isolate WI2 isolate

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag DSZ51_RS24800 Protein ID WP_000520549.1
Coordinates 1100..1348 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag DSZ51_RS24805 Protein ID WP_000681613.1
Coordinates 1345..1692 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSZ51_RS24790 55..354 + 300 WP_000650309.1 hypothetical protein -
DSZ51_RS24795 445..795 + 351 WP_000854259.1 hypothetical protein -
DSZ51_RS24800 1100..1348 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
DSZ51_RS24805 1345..1692 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DSZ51_RS24815 2053..2556 + 504 WP_000781813.1 hypothetical protein -
DSZ51_RS24820 2560..3789 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
DSZ51_RS24825 3866..4159 + 294 WP_060598542.1 LuxR family transcriptional regulator -
DSZ51_RS24830 4156..4371 + 216 WP_000760375.1 EexN family lipoprotein -
DSZ51_RS24835 4727..5347 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
DSZ51_RS24840 5415..5687 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33304 33304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T293553 WP_000520549.1 NZ_LT838201:1100-1348 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT293553 WP_000681613.1 NZ_LT838201:1345-1692 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References