Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4080713..4081289 Replicon chromosome
Accession NZ_LT838200
Organism Escherichia coli isolate WI2 isolate

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag DSZ51_RS21030 Protein ID WP_001297643.1
Coordinates 4081002..4081289 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag DSZ51_RS21025 Protein ID WP_000063156.1
Coordinates 4080713..4081015 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSZ51_RS21010 4077309..4079459 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
DSZ51_RS21015 4079509..4079712 + 204 WP_000467859.1 YbdD/YjiX family protein -
DSZ51_RS21020 4079723..4080679 + 957 WP_001297640.1 GTPase -
DSZ51_RS21025 4080713..4081015 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
DSZ51_RS21030 4081002..4081289 - 288 WP_001297643.1 BrnT family toxin Toxin
DSZ51_RS21035 4081488..4082185 - 698 Protein_3869 IS1 family transposase -
DSZ51_RS21040 4082242..4082337 + 96 Protein_3870 dienelactone hydrolase family protein -
DSZ51_RS21045 4082483..4083715 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
DSZ51_RS21050 4083756..4085036 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4081488..4081697 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T293550 WP_001297643.1 NZ_LT838200:c4081289-4081002 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT293550 WP_000063156.1 NZ_LT838200:c4081015-4080713 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References