Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4341125..4341622 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag WI2_RS22440 Protein ID WP_001173343.1
Coordinates 4341125..4341298 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag WI2_RS22445 Protein ID WP_000398619.1
Coordinates 4341326..4341622 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WI2_RS22430 4337795..4339252 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
WI2_RS22435 4339489..4341006 + 1518 WP_001295074.1 lysine--tRNA ligase -
WI2_RS22440 4341125..4341298 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
WI2_RS22445 4341326..4341622 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
WI2_RS22455 4341849..4342121 - 273 WP_000405651.1 N-acetyltransferase -
WI2_RS22460 4342133..4342363 - 231 WP_000371702.1 (4Fe-4S)-binding protein -
WI2_RS22465 4342544..4344175 + 1632 WP_001216477.1 sensor histidine kinase -
WI2_RS22470 4344172..4344891 + 720 WP_000611289.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T293527 WP_001173343.1 NZ_LT838196:c4341298-4341125 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT293527 WP_000398619.1 NZ_LT838196:c4341622-4341326 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References