Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4077819..4078395 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag WI2_RS20980 Protein ID WP_001297643.1
Coordinates 4078108..4078395 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag WI2_RS20975 Protein ID WP_000063156.1
Coordinates 4077819..4078121 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WI2_RS20960 4074415..4076565 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
WI2_RS20965 4076615..4076818 + 204 WP_000467859.1 YbdD/YjiX family protein -
WI2_RS20970 4076829..4077785 + 957 WP_001297640.1 GTPase -
WI2_RS20975 4077819..4078121 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
WI2_RS20980 4078108..4078395 - 288 WP_001297643.1 BrnT family toxin Toxin
WI2_RS20985 4078594..4079291 - 698 Protein_3868 IS1 family transposase -
WI2_RS20990 4079348..4079443 + 96 Protein_3869 dienelactone hydrolase family protein -
WI2_RS20995 4079589..4080821 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
WI2_RS21000 4080862..4082142 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T293525 WP_001297643.1 NZ_LT838196:c4078395-4078108 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT293525 WP_000063156.1 NZ_LT838196:c4078121-4077819 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References