Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1003248..1003831 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag WI2_RS05135 Protein ID WP_000254738.1
Coordinates 1003496..1003831 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag WI2_RS05130 Protein ID WP_000581937.1
Coordinates 1003248..1003496 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WI2_RS05120 999587..1000888 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
WI2_RS05125 1000936..1003170 + 2235 WP_000226815.1 GTP pyrophosphokinase -
WI2_RS05130 1003248..1003496 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
WI2_RS05135 1003496..1003831 + 336 WP_000254738.1 endoribonuclease MazF Toxin
WI2_RS05140 1003902..1004693 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
WI2_RS05145 1004921..1006558 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
WI2_RS05150 1006646..1007944 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T293512 WP_000254738.1 NZ_LT838196:1003496-1003831 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT293512 WP_000581937.1 NZ_LT838196:1003248-1003496 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References