Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 866552..867206 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag WI2_RS04500 Protein ID WP_000244777.1
Coordinates 866799..867206 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag WI2_RS04495 Protein ID WP_000354046.1
Coordinates 866552..866818 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WI2_RS04470 861721..862464 + 744 WP_000951948.1 SDR family oxidoreductase -
WI2_RS04475 862521..863954 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
WI2_RS04480 863999..864310 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
WI2_RS04485 864474..865133 + 660 WP_000250274.1 hemolysin III family protein -
WI2_RS04490 865329..866309 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
WI2_RS04495 866552..866818 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
WI2_RS04500 866799..867206 + 408 WP_000244777.1 toxin CptA Toxin
WI2_RS04505 867246..867767 - 522 WP_001055874.1 flavodoxin FldB -
WI2_RS04510 867879..868775 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
WI2_RS04515 868800..869510 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
WI2_RS04520 869516..871249 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T293511 WP_000244777.1 NZ_LT838196:866799-867206 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT293511 WP_000354046.1 NZ_LT838196:866552-866818 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References