Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4583792..4584289 Replicon chromosome
Accession NZ_LT827011
Organism Escherichia coli O127:H6 isolate EPEC E2348/69 variety

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BQ9544_RS24240 Protein ID WP_001173343.1
Coordinates 4584116..4584289 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BQ9544_RS24235 Protein ID WP_000398619.1
Coordinates 4583792..4584088 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9544_RS24215 4580522..4581241 - 720 WP_000611288.1 two-component system response regulator DcuR -
BQ9544_RS24220 4581238..4582869 - 1632 WP_001216494.1 sensor histidine kinase -
BQ9544_RS24225 4583050..4583280 + 231 WP_000371707.1 (4Fe-4S)-binding protein -
BQ9544_RS24230 4583292..4583564 + 273 WP_000405647.1 N-acetyltransferase -
BQ9544_RS24235 4583792..4584088 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BQ9544_RS24240 4584116..4584289 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BQ9544_RS24245 4584408..4585925 - 1518 WP_000003801.1 lysine--tRNA ligase -
BQ9544_RS24250 4586162..4587619 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T293507 WP_001173343.1 NZ_LT827011:4584116-4584289 [Escherichia coli O127:H6]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT293507 WP_000398619.1 NZ_LT827011:4583792-4584088 [Escherichia coli O127:H6]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References