Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3753924..3754555 Replicon chromosome
Accession NZ_LT827011
Organism Escherichia coli O127:H6 isolate EPEC E2348/69 variety

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag BQ9544_RS20120 Protein ID WP_001260301.1
Coordinates 3754280..3754555 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BQ9544_RS20115 Protein ID WP_000593555.1
Coordinates 3753924..3754283 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9544_RS20090 3750056..3751000 + 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
BQ9544_RS20095 3750997..3751830 + 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
BQ9544_RS20100 3751830..3752594 + 765 WP_001136232.1 nickel import ATP-binding protein NikD -
BQ9544_RS20105 3752591..3753397 + 807 WP_000173679.1 nickel import ATP-binding protein NikE -
BQ9544_RS20110 3753403..3753804 + 402 WP_001190063.1 nickel-responsive transcriptional regulator NikR -
BQ9544_RS20115 3753924..3754283 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BQ9544_RS20120 3754280..3754555 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
BQ9544_RS20125 3754628..3755752 - 1125 WP_001314210.1 ABC transporter permease -
BQ9544_RS20130 3755752..3758487 - 2736 WP_000149096.1 ribosome-associated ATPase/putative transporter RbbA -
BQ9544_RS20135 3758484..3759551 - 1068 WP_000361455.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T293504 WP_001260301.1 NZ_LT827011:c3754555-3754280 [Escherichia coli O127:H6]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT293504 WP_000593555.1 NZ_LT827011:c3754283-3753924 [Escherichia coli O127:H6]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References