Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1048399..1048982 Replicon chromosome
Accession NZ_LT795502
Organism Escherichia coli strain KV7 isolate KV7

Toxin (Protein)


Gene name mazF Uniprot ID U9Y5H3
Locus tag KJB12_RS04980 Protein ID WP_000254735.1
Coordinates 1048647..1048982 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U9Y5F1
Locus tag KJB12_RS04975 Protein ID WP_000581936.1
Coordinates 1048399..1048647 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJB12_RS04965 (1044738) 1044738..1046039 + 1302 WP_000046797.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KJB12_RS04970 (1046087) 1046087..1048321 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KJB12_RS04975 (1048399) 1048399..1048647 + 249 WP_000581936.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KJB12_RS04980 (1048647) 1048647..1048982 + 336 WP_000254735.1 endoribonuclease MazF Toxin
KJB12_RS04985 (1049054) 1049054..1049845 + 792 WP_001071673.1 nucleoside triphosphate pyrophosphohydrolase -
KJB12_RS04990 (1050073) 1050073..1051710 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KJB12_RS04995 (1051798) 1051798..1053096 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12070.03 Da        Isoelectric Point: 8.2605

>T293463 WP_000254735.1 NZ_LT795502:1048647-1048982 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9341.71 Da        Isoelectric Point: 4.4536

>AT293463 WP_000581936.1 NZ_LT795502:1048399-1048647 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHDNIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Y5H3

Antitoxin

Source ID Structure
AlphaFold DB U9Y5F1

References