Detailed information of TA system TA293404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2766794..2768250
• Replicon: chromosome
• Accession: NZ_LT632614
• Organism: Legionella pneumophila strain ST62 isolate ST62

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: BT141_RS12660
• Protein ID: WP_014842352.1
• Coordinates: 2766794..2767645 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: BT141_RS12665
• Protein ID: WP_027265135.1
• Coordinates: 2767642..2768250 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT141_RS12625	2761985..2762338	-	354	WP_061690731.1	conjugal transfer transcriptional regulator TraJ	-
BT141_RS12630	2762753..2763097	+	345	WP_014842348.1	TraK family protein	-
BT141_RS12635	2763097..2763822	+	726	WP_014842349.1	conjugal transfer protein TraL	-
BT141_RS12640	2763822..2764262	+	441	WP_042754801.1	conjugal transfer protein TraM	-
BT141_RS12645	2764427..2765884	+	1458	WP_061690732.1	putative DNA binding domain-containing protein	-
BT141_RS16375	2766205..2766285	-	81	WP_072356411.1	transposase	-
BT141_RS12655	2766294..2766713	+	420	Protein_2432	DUF2779 domain-containing protein	-
BT141_RS12660	2766794..2767645	-	852	WP_014842352.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BT141_RS12665	2767642..2768250	-	609	WP_027265135.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
BT141_RS12670	2768493..2768756	+	264	WP_014842354.1	hypothetical protein	-
BT141_RS12675	2768756..2769517	+	762	WP_042754800.1	DUF3800 domain-containing protein	-
BT141_RS12680	2769925..2771073	+	1149	WP_014842362.1	outer membrane beta-barrel protein	-
BT141_RS12685	2771103..2772221	+	1119	WP_014842363.1	DUF3494 domain-containing protein	-
BT141_RS16380	2772525..2772683	+	159	WP_154080660.1	hypothetical protein	-
BT141_RS12690	2772731..2773072	+	342	WP_027265134.1	IS630 transposase-related protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2714830..2801183	86353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31702.65 Da
• Isoelectric Point: 6.2785

>T293404 WP_014842352.1 NZ_LT632614:c2767645-2766794 [Legionella pneumophila]
MIKNIGESVRSRLKNIAVKEGSDFNAVLTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSIFADDGISFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLS
DLPAPKIRTYPIYTVIAEKLHAIALLGMANGRLKDYLDLYVLLGNEQIDNQILAKAIQATFNRRGMDLPEALPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22977.01 Da
• Isoelectric Point: 9.6557

>AT293404 WP_027265135.1 NZ_LT632614:c2768250-2767642 [Legionella pneumophila]
MKTEVNNKQKVLQMASKTGVIRMSDLTKKGITRATISRLVSENKLEKLAPGLYCLPETEFSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYNRAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVIVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp