Detailed information of TA system TA293333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3493442..3495297
• Replicon: chromosome
• Accession: NZ_LT615367
• Organism: Dickeya aquatica strain 174/2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A375ADW3
• Locus tag: DAQ1742_RS15850
• Protein ID: WP_035346276.1
• Coordinates: 3493951..3495297 (-)
• Length: 449 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: DAQ1742_RS15845
• Protein ID: WP_145916254.1
• Coordinates: 3493442..3493873 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAQ1742_RS15820	3488726..3489565	-	840	WP_035343794.1	MurR/RpiR family transcriptional regulator	-
DAQ1742_RS15825	3489792..3491381	+	1590	WP_035343796.1	gamma-glutamyltransferase family protein	-
DAQ1742_RS15830	3491406..3491603	+	198	WP_035343797.1	oxalurate catabolism protein HpxX	-
DAQ1742_RS15835	3491600..3492997	+	1398	WP_035343799.1	AtzE family amidohydrolase	-
DAQ1742_RS15840	3493008..3493388	+	381	WP_035343801.1	oxalurate catabolism protein HpxZ	-
DAQ1742_RS15845	3493442..3493873	-	432	WP_145916254.1	helix-turn-helix domain-containing protein	Antitoxin
DAQ1742_RS15850	3493951..3495297	-	1347	WP_035346276.1	type II toxin-antitoxin system HipA family toxin	Toxin
DAQ1742_RS15855	3495546..3496493	-	948	WP_180706173.1	allantoinase PuuE	-
DAQ1742_RS15860	3496571..3497308	-	738	WP_035343805.1	aspartate/glutamate racemase family protein	-
DAQ1742_RS15865	3497305..3498030	-	726	WP_035343806.1	GntR family transcriptional regulator	-
DAQ1742_RS15870	3498211..3499686	+	1476	WP_145916255.1	NCS1 family nucleobase:cation symporter-1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3499863..3500201	338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-389)

Antitoxin

• Cro/C1-type helix-turn-helix domain (64-116)

Sequences

Toxin

Protein

• Length: 449 a.a.
• Molecular weight: 50588.26 Da
• Isoelectric Point: 5.3179

>T293333 WP_035346276.1 NZ_LT615367:c3495297-3493951 [Dickeya aquatica]
MERLTVQAFIDNAWQDIAEIGFPESYRDNYLLTRLDYASDYGIDYMDCDDHHAVSVNYPVQIFFDDGGEPGWMRFLDDII
PAGASRQYWIEHLDLSGLPTQLQNYVLLRHATIAPVGHLRIKESIPAQHNGTTRFFSVEDVKNRASDFLDYAQQRGAAAG
GATGAGGEAPKLLLRCNADNRVWIDTWQDDLGCCDAAYLVKFPRGQRSEIDCDILRAEFHYYHELAEMGFDTIPVAGMRL
EEGRHYPSLWLPRFDIEFSGNGQVKRYGMESVYSLLRAGPGSALRHDRTIRTLIAKIVSSHTVSVDGKAFDPAAFVIEWI
RRDLLNIAFGNSDNHGRNTAFLRDERGIRLAPIYDFAPMKADPAGIARTTKWPEPLEIGGDYQFERIASLFSDILPPELL
MAKLQQTATQLVGLKARLQRRGVPESIITLPSIGFDYLPEKLQRWGVL

Nucleotide

• Length: 1347 bp

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15694.04 Da
• Isoelectric Point: 10.5131

>AT293333 WP_145916254.1 NZ_LT615367:c3493873-3493442 [Dickeya aquatica]
MQERSVAQTYFQPSSVAVVTETADITKVRASKNSRPLDTSERRLAMQNVIRRMMFGEISQGNALKELRLNVLGLRQEAYA
TLVGVSRKTLSEIENGKGNYTADIINKVFKPFGLKMGLMPVSPSLLTEILSTAPTSGGQSDSR

Nucleotide

• Length: 432 bp

Structures

Toxin

• AlphaFold DB: A0A375ADW3