Detailed information of TA system TA293294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-higA/BrnT_toxin-HTH
• Location: 437324..437921
• Replicon: chromosome
• Accession: NZ_LT606946
• Organism: Polynucleobacter necessarius isolate PPGSP5

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: DXE44_RS02350
• Protein ID: WP_114652349.1
• Coordinates: 437324..437602 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXE44_RS10230
• Protein ID: WP_197712901.1
• Coordinates: 437718..437921 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE44_RS02325	432883..433401	+	519	WP_114652338.1	response regulator transcription factor	-
DXE44_RS02330	433486..434526	+	1041	WP_114652340.1	helix-turn-helix domain-containing protein	-
DXE44_RS02335	434561..434899	-	339	WP_114652342.1	P-II family nitrogen regulator	-
DXE44_RS02340	434951..436570	-	1620	WP_114652345.1	NAD+ synthase	-
DXE44_RS02345	436588..437124	-	537	WP_114652347.1	inorganic diphosphatase	-
DXE44_RS02350	437324..437602	+	279	WP_114652349.1	BrnT family toxin	Toxin
DXE44_RS10230	437718..437921	+	204	WP_197712901.1	helix-turn-helix domain-containing protein	Antitoxin
DXE44_RS02360	437989..438945	-	957	WP_114652353.1	thioredoxin-disulfide reductase	-
DXE44_RS02365	438985..441297	+	2313	WP_114652355.1	DNA translocase FtsK	-
DXE44_RS02370	441257..442000	+	744	WP_197712808.1	outer membrane lipoprotein carrier protein LolA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-59)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11122.66 Da
• Isoelectric Point: 7.9955

>T293294 WP_114652349.1 NZ_LT606946:437324-437602 [Polynucleobacter necessarius]
MDFEWDMAKSDLCQISRNFDFTYVVSVFIDLNLLIERDQRWDYGEERFRALGLLSEKVFVVIYTKRPAAIRIISARRANR
REVRRYEENHSS

Nucleotide

• Length: 279 bp

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7436.65 Da
• Isoelectric Point: 10.3534

>AT293294 WP_197712901.1 NZ_LT606946:437718-437921 [Polynucleobacter necessarius]
MLDAAQYAKGVRERIGLSQQEFSKRIEVSLETIRNWEQGKRSPTGAAKALLKILDRAPEIALLALSV

Nucleotide

• Length: 204 bp