Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5091019..5091595 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NCTC86EC_RS25075 Protein ID WP_001297643.1
Coordinates 5091019..5091306 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NCTC86EC_RS25080 Protein ID WP_000063156.1
Coordinates 5091293..5091595 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS25050 5087256..5088536 - 1281 WP_001037390.1 DUF445 domain-containing protein -
NCTC86EC_RS25055 5088577..5089809 - 1233 WP_001137032.1 multidrug efflux MFS transporter MdtM -
NCTC86EC_RS25070 5090124..5090820 + 697 Protein_4758 IS1 family transposase -
NCTC86EC_RS25075 5091019..5091306 + 288 WP_001297643.1 BrnT family toxin Toxin
NCTC86EC_RS25080 5091293..5091595 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NCTC86EC_RS25085 5091629..5092585 - 957 WP_001297640.1 GTPase -
NCTC86EC_RS25090 5092596..5092799 - 204 WP_000467860.1 YbdD/YjiX family protein -
NCTC86EC_RS25095 5092849..5094999 - 2151 WP_066009113.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5090611..5090820 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T293283 WP_001297643.1 NZ_LT601384:5091019-5091306 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT293283 WP_000063156.1 NZ_LT601384:5091293-5091595 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References