Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4753819..4754316 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NCTC86EC_RS23310 Protein ID WP_001173343.1
Coordinates 4754143..4754316 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NCTC86EC_RS23305 Protein ID WP_000398619.1
Coordinates 4753819..4754115 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS23285 4750550..4751269 - 720 WP_000611288.1 two-component system response regulator DcuR -
NCTC86EC_RS23290 4751266..4752897 - 1632 WP_066009715.1 sensor histidine kinase -
NCTC86EC_RS23295 4753078..4753308 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
NCTC86EC_RS23300 4753320..4753592 + 273 WP_000405651.1 N-acetyltransferase -
NCTC86EC_RS23305 4753819..4754115 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NCTC86EC_RS23310 4754143..4754316 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NCTC86EC_RS23315 4754435..4755952 - 1518 WP_066009713.1 lysine--tRNA ligase -
NCTC86EC_RS23320 4756189..4757646 - 1458 WP_066009710.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T293280 WP_001173343.1 NZ_LT601384:4754143-4754316 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T293280 NZ_LT601384:4754143-4754316 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT293280 WP_000398619.1 NZ_LT601384:4753819-4754115 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT293280 NZ_LT601384:4753819-4754115 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References