Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3327405..3328059 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NCTC86EC_RS16355 Protein ID WP_000244777.1
Coordinates 3327405..3327812 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NCTC86EC_RS16360 Protein ID WP_000354046.1
Coordinates 3327793..3328059 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS16335 3323362..3325095 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
NCTC86EC_RS16340 3325101..3325811 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NCTC86EC_RS16345 3325836..3326732 - 897 WP_042091714.1 site-specific tyrosine recombinase XerD -
NCTC86EC_RS16350 3326844..3327365 + 522 WP_001055874.1 flavodoxin FldB -
NCTC86EC_RS16355 3327405..3327812 - 408 WP_000244777.1 toxin CptA Toxin
NCTC86EC_RS16360 3327793..3328059 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NCTC86EC_RS16365 3328302..3329282 + 981 WP_001514522.1 tRNA-modifying protein YgfZ -
NCTC86EC_RS16370 3329478..3330137 - 660 WP_000250274.1 hemolysin III family protein -
NCTC86EC_RS16375 3330301..3330612 - 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
NCTC86EC_RS16380 3330657..3332090 + 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T293275 WP_000244777.1 NZ_LT601384:c3327812-3327405 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT293275 WP_000354046.1 NZ_LT601384:c3328059-3327793 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References