Detailed information of TA system TA293271

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2913372..2914066
• Replicon: chromosome
• Accession: NZ_LT601384
• Organism: Escherichia coli isolate NCTC86EC

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: NCTC86EC_RS14310
• Protein ID: WP_066009356.1
• Coordinates: 2913372..2913740 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: NCTC86EC_RS14315
• Protein ID: WP_077249034.1
• Coordinates: 2913761..2914066 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCTC86EC_RS14290	2908545..2909501	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
NCTC86EC_RS14295	2909498..2911696	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
NCTC86EC_RS14300	2911706..2912662	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
NCTC86EC_RS14305	2912713..2913051	+	339	Protein_2737	LysR family transcriptional regulator	-
NCTC86EC_RS14310	2913372..2913740	-	369	WP_066009356.1	TA system toxin CbtA family protein	Toxin
NCTC86EC_RS14315	2913761..2914066	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NCTC86EC_RS14320	2914170..2914814	-	645	WP_000086755.1	hypothetical protein	-
NCTC86EC_RS14325	2914833..2915054	-	222	WP_000692345.1	DUF987 domain-containing protein	-
NCTC86EC_RS14330	2915123..2915599	-	477	WP_001424026.1	RadC family protein	-
NCTC86EC_RS14335	2915615..2916088	-	474	WP_000855059.1	antirestriction protein	-
NCTC86EC_RS14340	2916430..2917251	-	822	WP_001234565.1	DUF945 domain-containing protein	-
NCTC86EC_RS28550	2917352..2917560	-	209	Protein_2745	DUF905 family protein	-
NCTC86EC_RS14350	2917661..2918116	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	2887999..2938777	50778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13611.75 Da
• Isoelectric Point: 7.7465

>T293271 WP_066009356.1 NZ_LT601384:c2913740-2913372 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDASITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT293271 WP_077249034.1 NZ_LT601384:c2914066-2913761 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp