Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2718662..2719280 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag NCTC86EC_RS13365 Protein ID WP_001291435.1
Coordinates 2719062..2719280 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag NCTC86EC_RS13360 Protein ID WP_000344800.1
Coordinates 2718662..2719036 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS13350 2713751..2714944 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
NCTC86EC_RS13355 2714967..2718116 + 3150 WP_066009150.1 efflux RND transporter permease AcrB -
NCTC86EC_RS13360 2718662..2719036 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
NCTC86EC_RS13365 2719062..2719280 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
NCTC86EC_RS13370 2719452..2720003 + 552 WP_000102564.1 maltose O-acetyltransferase -
NCTC86EC_RS13375 2720119..2720589 + 471 WP_000136192.1 YlaC family protein -
NCTC86EC_RS13380 2720753..2722303 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
NCTC86EC_RS13385 2722345..2722698 - 354 WP_000878140.1 DUF1428 domain-containing protein -
NCTC86EC_RS13390 2723042..2723269 - 228 WP_024183738.1 DUF2188 domain-containing protein -
NCTC86EC_RS13395 2723487..2724107 - 621 WP_001267012.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T293270 WP_001291435.1 NZ_LT601384:2719062-2719280 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT293270 WP_000344800.1 NZ_LT601384:2718662-2719036 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References