293269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2142594..2144314 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag NCTC86EC_RS10520 Protein ID WP_000312833.1
Coordinates 2142594..2143250 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag NCTC86EC_RS10525 Protein ID WP_000634203.1
Coordinates 2143247..2144314 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS28830 2137995..2138249 - 255 WP_154670580.1 hypothetical protein -
NCTC86EC_RS10505 2138531..2138770 + 240 WP_001431807.1 hypothetical protein -
NCTC86EC_RS10510 2138810..2141248 + 2439 WP_000985465.1 type I restriction-modification system subunit M -
NCTC86EC_RS10515 2141248..2142581 + 1334 Protein_2006 restriction endonuclease subunit S -
NCTC86EC_RS10520 2142594..2143250 + 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
NCTC86EC_RS10525 2143247..2144314 + 1068 WP_000634203.1 macro domain-containing protein Antitoxin
NCTC86EC_RS10530 2144333..2147428 + 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
NCTC86EC_RS10535 2147428..2148144 + 717 WP_001122107.1 M48 family metallopeptidase -
NCTC86EC_RS10540 2148489..2148668 + 180 Protein_2011 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T293269 WP_000312833.1 NZ_LT601384:2142594-2143250 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT293269 WP_000634203.1 NZ_LT601384:2143247-2144314 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References