Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 543722..544293 Replicon chromosome
Accession NZ_LT598663
Organism Enterococcus faecium isolate Ef_aus00233

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag BN9748_RS02750 Protein ID WP_002286801.1
Coordinates 543952..544293 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag BN9748_RS02745 Protein ID WP_002323011.1
Coordinates 543722..543952 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN9748_RS02705 538965..540296 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
BN9748_RS02710 540318..540944 + 627 WP_002333420.1 cysteine hydrolase -
BN9748_RS02715 541127..541708 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
BN9748_RS02730 542317..542892 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
BN9748_RS02735 543097..543435 - 339 WP_002286804.1 hypothetical protein -
BN9748_RS02745 543722..543952 + 231 WP_002323011.1 hypothetical protein Antitoxin
BN9748_RS02750 543952..544293 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BN9748_RS02755 545143..545328 + 186 WP_002304207.1 hypothetical protein -
BN9748_RS02760 545833..546027 + 195 WP_002295146.1 hypothetical protein -
BN9748_RS02765 546185..546467 + 283 Protein_519 transposase -
BN9748_RS02770 546711..547541 - 831 WP_002286789.1 manganese catalase family protein -
BN9748_RS02775 547749..549083 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T293241 WP_002286801.1 NZ_LT598663:543952-544293 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT293241 WP_002323011.1 NZ_LT598663:543722-543952 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References