Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4545097..4545897 Replicon chromosome
Accession NZ_LT594504
Organism Escherichia coli isolate E. coli RL465

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag RL465_RS22010 Protein ID WP_000342449.1
Coordinates 4545097..4545624 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag RL465_RS22015 Protein ID WP_001277108.1
Coordinates 4545631..4545897 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RL465_RS21985 4540172..4540939 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
RL465_RS21990 4540936..4542213 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
RL465_RS21995 4542210..4543136 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
RL465_RS22000 4543184..4544293 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
RL465_RS22005 4544717..4545100 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
RL465_RS22010 4545097..4545624 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
RL465_RS22015 4545631..4545897 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
RL465_RS22020 4546047..4547150 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
RL465_RS22025 4547422..4548276 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
RL465_RS22030 4548521..4549579 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
RL465_RS22035 4549572..4550240 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T293228 WP_000342449.1 NZ_LT594504:c4545624-4545097 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT293228 WP_001277108.1 NZ_LT594504:c4545897-4545631 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References