Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 661588..662256 Replicon draft genome
Accession NC_021006
Organism Streptococcus pneumoniae SPN034156

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag SPN034156_RS03500 Protein ID WP_001132284.1
Coordinates 662077..662256 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag SPN034156_RS03495 Protein ID WP_000961810.1
Coordinates 661588..662040 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPN034156_RS03470 657478..658221 - 744 WP_001188199.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
SPN034156_RS03475 658223..659173 - 951 WP_000451132.1 50S ribosomal protein L11 methyltransferase -
SPN034156_RS03480 659311..659739 - 429 WP_000418176.1 NUDIX hydrolase -
SPN034156_RS03485 659705..660808 - 1104 WP_000719717.1 M50 family metallopeptidase -
SPN034156_RS03490 660827..661297 - 471 WP_000257106.1 DUF3013 family protein -
SPN034156_RS03495 661588..662040 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SPN034156_RS03500 662077..662256 - 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
SPN034156_RS03505 662393..662572 - 180 WP_001809903.1 hypothetical protein -
SPN034156_RS03510 662737..662976 - 240 WP_000818078.1 hypothetical protein -
SPN034156_RS03515 663246..664517 + 1272 WP_001113196.1 replication-associated recombination protein A -
SPN034156_RS03525 665062..666381 - 1320 WP_000502545.1 glycoside hydrolase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T29320 WP_001132284.1 NC_021006:c662256-662077 [Streptococcus pneumoniae SPN034156]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T29320 NC_021006:c662256-662077 [Streptococcus pneumoniae SPN034156]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT29320 WP_000961810.1 NC_021006:c662040-661588 [Streptococcus pneumoniae SPN034156]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT29320 NC_021006:c662040-661588 [Streptococcus pneumoniae SPN034156]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References