Detailed information of TA system TA293151

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2501984..2502699
• Replicon: chromosome
• Accession: NZ_LT556085
• Organism: Citrobacter amalonaticus strain 92

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: CITRO92_RS12475
• Protein ID: WP_023303149.1
• Coordinates: 2502331..2502699 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: CITRO92_RS12470
• Protein ID: WP_023303148.1
• Coordinates: 2501984..2502310 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CITRO92_RS12440	2497018..2498283	+	1266	WP_023303143.1	hypothetical protein	-
CITRO92_RS12445	2498592..2499026	+	435	WP_023303144.1	hypothetical protein	-
CITRO92_RS12450	2499085..2499924	+	840	WP_023303145.1	hypothetical protein	-
CITRO92_RS12460	2500614..2501435	+	822	WP_023303146.1	DUF945 domain-containing protein	-
CITRO92_RS12465	2501501..2501971	+	471	WP_023303147.1	DNA repair protein RadC	-
CITRO92_RS12470	2501984..2502310	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CITRO92_RS12475	2502331..2502699	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
CITRO92_RS12480	2503268..2503633	-	366	WP_109739897.1	TA system toxin CbtA family protein	-
CITRO92_RS12485	2503669..2504007	-	339	WP_109739898.1	type IV toxin-antitoxin system YeeU family antitoxin	-
CITRO92_RS12490	2504023..2504244	-	222	WP_109739899.1	DUF987 family protein	-
CITRO92_RS12495	2504241..2504741	-	501	WP_174217526.1	DNA repair protein RadC	-
CITRO92_RS12500	2504771..2505589	-	819	WP_109739900.1	DUF945 domain-containing protein	-
CITRO92_RS12505	2505689..2506366	-	678	WP_109739901.1	hypothetical protein	-
CITRO92_RS12510	2506653..2507546	-	894	WP_109739902.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2498592..2520889	22297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T293151 WP_023303149.1 NZ_LT556085:2502331-2502699 [Citrobacter amalonaticus]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT293151 WP_023303148.1 NZ_LT556085:2501984-2502310 [Citrobacter amalonaticus]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp