Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 76359..76939 Replicon plasmid p58T1
Accession NZ_LT222313
Organism Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf

Toxin (Protein)


Gene name PumA Uniprot ID A0A2G4CV40
Locus tag PCPL58_RS00390 Protein ID WP_044322659.1
Coordinates 76640..76939 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A2G4CUT8
Locus tag PCPL58_RS00385 Protein ID WP_003407168.1
Coordinates 76359..76643 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCPL58_RS00360 72564..73649 - 1086 WP_002556026.1 DUF3616 domain-containing protein -
PCPL58_RS00365 73800..74021 + 222 WP_004666801.1 hypothetical protein -
PCPL58_RS28560 74585..75603 - 1019 Protein_76 tyrosine protein phosphatase -
PCPL58_RS00380 75976..76284 + 309 WP_181135240.1 DUF4113 domain-containing protein -
PCPL58_RS00385 76359..76643 - 285 WP_003407168.1 putative addiction module antidote protein Antitoxin
PCPL58_RS00390 76640..76939 - 300 WP_044322659.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PCPL58_RS00395 77449..77865 + 417 WP_044322716.1 hypothetical protein -
PCPL58_RS30395 77874..78107 - 234 WP_134931282.1 hypothetical protein -
PCPL58_RS00405 78261..78914 + 654 WP_044322644.1 AAA family ATPase -
PCPL58_RS00410 79003..79254 + 252 WP_003348789.1 ribbon-helix-helix protein, CopG family -
PCPL58_RS00415 79518..80135 + 618 WP_065348578.1 SOS response-associated peptidase -
PCPL58_RS28570 80326..81004 + 679 Protein_85 tyrosine-type recombinase/integrase -
PCPL58_RS00425 81067..81876 - 810 WP_005741073.1 IS21-like element ISPsy4 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..101345 101345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-95)

Antitoxin

Uncharacterized protein HI1420

(10-92)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11170.80 Da        Isoelectric Point: 9.9231

>T293120 WP_044322659.1 NZ_LT222313:c76939-76640 [Pseudomonas cerasi]
MTTIKQTSTYMAWERRLKDQKAKAAIAARIFRVANGLMGDVSPVGQGVSELRIHVGPGYRVYFQQRGDELILLLCGGDKS
SQSRDIETAKKLADQWWQE

Download         Length: 300 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10277.83 Da        Isoelectric Point: 5.0916

>AT293120 WP_003407168.1 NZ_LT222313:c76643-76359 [Pseudomonas cerasi]
MKEQIFDYDPAAALDGPEAIAIFISDAFETGDAEYIAKAMGVVARAKGMTELARETGLSREQLYRSFSSKGNPTLKTMLT
VMRALGVDMTARMH

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G4CV40

Antitoxin

Source ID Structure
AlphaFold DB A0A2G4CUT8

References