Detailed information of TA system TA029312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1016015..1016649
• Replicon: chromosome
• Accession: NC_020995
• Organism: Enterococcus casseliflavus EC20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C9A6C2
• Locus tag: ECBG_RS04570
• Protein ID: WP_015509249.1
• Coordinates: 1016299..1016649 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ECBG_RS04565
• Protein ID: WP_080583835.1
• Coordinates: 1016015..1016305 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBG_RS04555	1011641..1014034	+	2394	WP_015509246.1	DUF4982 domain-containing protein	-
ECBG_RS04560	1014171..1015391	+	1221	WP_015509247.1	MFS transporter	-
ECBG_RS04565	1016015..1016305	+	291	WP_080583835.1	PbsX family transcriptional regulator	Antitoxin
ECBG_RS04570	1016299..1016649	+	351	WP_015509249.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ECBG_RS04575	1016964..1017389	-	426	WP_034869932.1	AsnC family transcriptional regulator	-
ECBG_RS04580	1017492..1018037	+	546	WP_017144326.1	cysteine hydrolase	-
ECBG_RS04590	1018353..1019276	+	924	WP_026008044.1	peptide-methionine (S)-S-oxide reductase MsrA	-
ECBG_RS04595	1019626..1020987	+	1362	WP_015509253.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (13-96)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13007.96 Da
• Isoelectric Point: 7.3540

>T29312 WP_015509249.1 NC_020995:1016299-1016649 [Enterococcus casseliflavus EC20]
MVRNPRQGDILLLDTAPRAGHEQTGKRPYIVLSHDMIADYSNVATVAPISSTSRNYPLYVSINPDYGMKTTGKVLLDQLT
TIDYEASACVFLEKAHEHLVDELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T29312 NC_020995:1016299-1016649 [Enterococcus casseliflavus EC20]
ATGGTAAGAAATCCTAGACAAGGGGATATTTTGTTATTGGATACAGCTCCTCGTGCTGGACATGAACAAACGGGAAAAAG
GCCCTATATTGTTTTAAGTCATGACATGATTGCAGATTATAGTAACGTTGCCACCGTTGCTCCGATTTCGAGTACAAGTA
GAAACTATCCGTTATATGTAAGTATTAATCCAGATTATGGCATGAAGACAACAGGAAAAGTATTGTTGGATCAATTAACG
ACAATTGATTATGAAGCCAGCGCGTGTGTTTTTTTGGAAAAAGCGCATGAGCACTTGGTTGATGAGTTATTGATGAAAGT
TAGAACAGTATTTCAAAAAGTAAGTAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11265.84 Da
• Isoelectric Point: 4.9389

>AT29312 WP_080583835.1 NC_020995:1016015-1016305 [Enterococcus casseliflavus EC20]
MYIQKEVSNMEMLPIKKWGNSNGLRLPKYIMEYLGIHTDDKVKIIQEEVNGHKRLIIEAANVENELTIEELFENYTAERN
HVEIQHLGEAVGNEKW

Nucleotide

• Length: 291 bp

>AT29312 NC_020995:1016015-1016305 [Enterococcus casseliflavus EC20]
ATGTATATACAGAAAGAGGTGTCGAACATGGAAATGCTACCTATAAAAAAATGGGGCAATAGTAACGGTCTTAGATTGCC
AAAGTACATTATGGAGTATCTTGGCATTCATACTGATGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGACATAAAC
GGCTAATCATCGAAGCGGCAAATGTAGAAAACGAGTTAACGATTGAAGAGTTATTTGAAAATTACACTGCTGAGAGAAAT
CATGTGGAAATTCAACATTTAGGTGAAGCAGTAGGGAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: C9A6C2