Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2652972..2653623 Replicon chromosome
Accession NZ_LT220504
Organism Lacticaseibacillus rhamnosus strain BPL5

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag BN3853_RS12435 Protein ID WP_005686631.1
Coordinates 2653240..2653623 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag BN3853_RS12430 Protein ID WP_005686632.1
Coordinates 2652972..2653220 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN3853_RS12410 2648021..2649409 + 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
BN3853_RS12415 2649683..2651191 + 1509 WP_005686635.1 DEAD/DEAH box helicase -
BN3853_RS12420 2651360..2651734 + 375 WP_005686634.1 holo-ACP synthase -
BN3853_RS12425 2651721..2652860 + 1140 WP_005691215.1 alanine racemase -
BN3853_RS12430 2652972..2653220 + 249 WP_005686632.1 antitoxin Antitoxin
BN3853_RS12435 2653240..2653623 + 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BN3853_RS12445 2654137..2654664 + 528 WP_005691221.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T293116 WP_005686631.1 NZ_LT220504:2653240-2653623 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT293116 WP_005686632.1 NZ_LT220504:2652972-2653220 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References