Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42413..43056 Replicon plasmid 2
Accession NZ_LS999561
Organism Escherichia coli isolate EC-TO143

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag ECTO143_RS25140 Protein ID WP_001034044.1
Coordinates 42413..42829 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag ECTO143_RS25145 Protein ID WP_001261286.1
Coordinates 42826..43056 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO143_RS25115 37545..38459 - 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -
ECTO143_RS25125 38815..39512 + 698 Protein_40 IS1 family transposase -
ECTO143_RS25130 39766..40788 - 1023 WP_000361402.1 helicase UvrD -
ECTO143_RS25135 40773..42338 - 1566 WP_001128474.1 AAA family ATPase -
ECTO143_RS25140 42413..42829 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
ECTO143_RS25145 42826..43056 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECTO143_RS25150 43437..47231 + 3795 WP_001144732.1 hypothetical protein -
ECTO143_RS25155 47276..47692 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
ECTO143_RS25160 47689..47919 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD - 1..134692 134692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T293082 WP_001034044.1 NZ_LS999561:c42829-42413 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT293082 WP_001261286.1 NZ_LS999561:c43056-42826 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References