Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4213854..4214430 Replicon chromosome
Accession NZ_LS999560
Organism Escherichia coli isolate EC-TO143

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag ECTO143_RS20965 Protein ID WP_001295743.1
Coordinates 4214143..4214430 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag ECTO143_RS20960 Protein ID WP_000063148.1
Coordinates 4213854..4214156 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO143_RS20945 4210450..4212600 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
ECTO143_RS20950 4212650..4212853 + 204 WP_000467859.1 YbdD/YjiX family protein -
ECTO143_RS20955 4212864..4213820 + 957 WP_001295745.1 GTPase -
ECTO143_RS20960 4213854..4214156 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
ECTO143_RS20965 4214143..4214430 - 288 WP_001295743.1 BrnT family toxin Toxin
ECTO143_RS20970 4214627..4215793 + 1167 WP_000800831.1 restriction endonuclease -
ECTO143_RS20975 4215857..4217476 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
ECTO143_RS20980 4217466..4218770 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T293076 WP_001295743.1 NZ_LS999560:c4214430-4214143 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT293076 WP_000063148.1 NZ_LS999560:c4214156-4213854 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References