Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 287474..288274 Replicon chromosome
Accession NZ_LS999560
Organism Escherichia coli isolate EC-TO143

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag ECTO143_RS01345 Protein ID WP_000342452.1
Coordinates 287747..288274 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag ECTO143_RS01340 Protein ID WP_001277107.1
Coordinates 287474..287740 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO143_RS01320 283133..283801 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
ECTO143_RS01325 283794..284852 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
ECTO143_RS01330 285097..285951 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
ECTO143_RS01335 286222..287325 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
ECTO143_RS01340 287474..287740 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
ECTO143_RS01345 287747..288274 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
ECTO143_RS01350 288271..288654 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
ECTO143_RS01355 289077..290186 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
ECTO143_RS01360 290234..291160 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ECTO143_RS01365 291157..292434 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
ECTO143_RS01370 292431..293198 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T293060 WP_000342452.1 NZ_LS999560:287747-288274 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT293060 WP_001277107.1 NZ_LS999560:287474-287740 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References