Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77648..78291 Replicon plasmid 2
Accession NZ_LS998786
Organism Escherichia coli isolate EC-TO75

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag ECTO75_RS23655 Protein ID WP_001034044.1
Coordinates 77648..78064 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag ECTO75_RS23660 Protein ID WP_001261286.1
Coordinates 78061..78291 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO75_RS23640 74061..74747 + 687 Protein_88 IS1 family transposase -
ECTO75_RS23645 75001..76023 - 1023 WP_000361402.1 helicase UvrD -
ECTO75_RS23650 76008..77573 - 1566 WP_001128474.1 AAA family ATPase -
ECTO75_RS23655 77648..78064 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
ECTO75_RS23660 78061..78291 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECTO75_RS23670 78851..79069 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
ECTO75_RS23675 79071..79376 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
ECTO75_RS23680 79377..80183 + 807 WP_000016970.1 site-specific integrase -
ECTO75_RS23685 80905..81660 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD iucA / iucB / iucC / iucD / iutA 1..103198 103198
- flank IS/Tn - - 74400..74747 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T293033 WP_001034044.1 NZ_LS998786:c78064-77648 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT293033 WP_001261286.1 NZ_LS998786:c78291-78061 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References