Detailed information of TA system TA029303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 140673..141178
• Replicon: chromosome
• Accession: NC_020912
• Organism: Pseudomonas aeruginosa B136-33

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: G655_RS00630
• Protein ID: WP_003083773.1
• Coordinates: 140673..140954 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: G655_RS00635
• Protein ID: WP_003083775.1
• Coordinates: 140951..141178 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G655_RS00605	135924..137273	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
G655_RS00610	137322..138008	+	687	WP_003083762.1	FadR family transcriptional regulator	-
G655_RS00615	138109..138843	+	735	WP_003110658.1	GntR family transcriptional regulator	-
G655_RS00620	139047..139433	+	387	WP_003083767.1	aegerolysin family protein	-
G655_RS00625	139465..140373	-	909	WP_003083769.1	LysR family transcriptional regulator	-
G655_RS00630	140673..140954	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
G655_RS00635	140951..141178	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
G655_RS00640	141354..141977	-	624	WP_003137011.1	hypothetical protein	-
G655_RS00645	142078..142578	+	501	WP_003101228.1	LEA type 2 family protein	-
G655_RS00650	142650..142991	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
G655_RS00655	143073..144500	-	1428	WP_003083784.1	GABA permease	-
G655_RS00660	144669..146162	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T29303 WP_003083773.1 NC_020912:c140954-140673 [Pseudomonas aeruginosa B136-33]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T29303 NC_020912:c140954-140673 [Pseudomonas aeruginosa B136-33]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT29303 WP_003083775.1 NC_020912:c141178-140951 [Pseudomonas aeruginosa B136-33]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT29303 NC_020912:c141178-140951 [Pseudomonas aeruginosa B136-33]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9