Detailed information of TA system TA029302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 92488..93086
• Replicon: plasmid pOA238_118
• Accession: NC_020909
• Organism: Octadecabacter arcticus 238

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: M9RY08
• Locus tag: OA238_RS27440
• Protein ID: WP_015497696.1
• Coordinates: 92488..92742 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: M9RWB8
• Locus tag: OA238_RS27445
• Protein ID: WP_015497697.1
• Coordinates: 92739..93086 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OA238_RS27410	88116..88946	-	831	WP_015497692.1	replication initiation protein	-
OA238_RS27415	89613..90125	+	513	WP_015495668.1	IS630 family transposase	-
OA238_RS27420	90353..91312	+	960	WP_015496121.1	IS1595-like element ISOar1 family transposase	-
OA238_RS27425	91418..91636	+	219	WP_015497693.1	antitoxin MazE family protein	-
OA238_RS27430	91633..91959	+	327	WP_187293218.1	type II toxin-antitoxin system PemK/MazF family toxin	-
OA238_RS27435	91980..92291	-	312	WP_015497695.1	H-NS histone family protein	-
OA238_RS33260	92323..92472	-	150	WP_187293214.1	hypothetical protein	-
OA238_RS27440	92488..92742	+	255	WP_015497696.1	type II toxin-antitoxin system HicA family toxin	Toxin
OA238_RS27445	92739..93086	+	348	WP_015497697.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
OA238_RS27450	93280..93519	+	240	Protein_102	transposase	-
OA238_RS31100	94179..94995	-	817	Protein_103	IS5 family transposase	-
OA238_RS27465	95138..96114	-	977	Protein_104	IS1595 family transposase	-
OA238_RS27470	96201..96407	-	207	Protein_105	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118287	118287
	-	inside	IScluster/Tn	-	-	90353..103479	13126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-79)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9498.84 Da
• Isoelectric Point: 10.2653

>T29302 WP_015497696.1 NC_020909:92488-92742 [Octadecabacter arcticus 238]
MNNKHRKALATVFTDPVSGTVEWTAIEKLLIAADAEVVEGNGSRVRFVKDDEVETFHRPHPAKEAKRYQVRAARAFLERI
GVRP

Nucleotide

• Length: 255 bp

>T29302 NC_020909:92488-92742 [Octadecabacter arcticus 238]
ATGAACAACAAGCACCGCAAAGCACTTGCCACTGTTTTCACTGACCCAGTGTCAGGAACGGTAGAATGGACAGCGATTGA
AAAGCTACTCATCGCCGCTGATGCCGAGGTCGTTGAAGGCAACGGTTCGCGGGTGCGCTTCGTGAAGGATGACGAGGTTG
AGACGTTTCATCGTCCACACCCAGCCAAGGAAGCTAAGCGGTATCAAGTCCGCGCAGCTCGAGCGTTTTTGGAAAGGATA
GGGGTCAGGCCATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12685.08 Da
• Isoelectric Point: 4.9411

>AT29302 WP_015497697.1 NC_020909:92739-93086 [Octadecabacter arcticus 238]
MTNTMTYKGYSARIEYDDEDGIMTGQIAGIRDGVGFHADSVEELKDAFHEAVDDYVDTCAKIGKEPQKPYSGRVMFRVDP
EVHRKAALAAELSGKSLNQWAEEVLNRAAHSGHPA

Nucleotide

• Length: 348 bp

>AT29302 NC_020909:92739-93086 [Octadecabacter arcticus 238]
ATGACAAATACGATGACTTACAAAGGTTACTCTGCCCGCATCGAATATGACGATGAAGACGGCATCATGACCGGCCAGAT
TGCTGGCATCCGTGACGGCGTAGGCTTCCACGCAGACAGTGTGGAAGAGCTGAAAGACGCGTTCCATGAGGCCGTCGATG
ATTACGTCGATACATGCGCCAAGATAGGCAAGGAACCACAGAAGCCATACTCTGGCCGTGTGATGTTTCGCGTCGATCCA
GAGGTCCACCGCAAGGCTGCACTGGCAGCAGAGCTGTCAGGCAAAAGCCTGAACCAGTGGGCAGAAGAAGTTCTTAATCG
TGCGGCGCATAGTGGGCATCCGGCGTGA

Structures

Toxin

• AlphaFold DB: M9RY08

Antitoxin

• AlphaFold DB: M9RWB8