Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 99455..100098 Replicon plasmid 2
Accession NZ_LS992193
Organism Escherichia coli isolate Escherichia coli str. TO217

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag ECTO217_RS27455 Protein ID WP_001034044.1
Coordinates 99682..100098 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag ECTO217_RS27450 Protein ID WP_001261286.1
Coordinates 99455..99685 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO217_RS27425 96086..96841 - 756 WP_000852146.1 replication initiation protein RepE -
ECTO217_RS27430 97563..98369 - 807 WP_000016970.1 site-specific integrase -
ECTO217_RS27435 98370..98675 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
ECTO217_RS27440 98677..98895 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
ECTO217_RS27450 99455..99685 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECTO217_RS27455 99682..100098 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
ECTO217_RS27460 100173..101738 + 1566 WP_001128474.1 AAA family ATPase -
ECTO217_RS27465 101723..102745 + 1023 WP_000361402.1 helicase UvrD -
ECTO217_RS27470 102999..103685 - 687 Protein_128 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD 1..128948 128948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T292970 WP_001034044.1 NZ_LS992193:99682-100098 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT292970 WP_001261286.1 NZ_LS992193:99455-99685 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References