Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2776195..2776715 Replicon
Accession NC_020891
Organism Clavibacter michiganensis subsp. nebraskensis NCPPB 2581

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag CMN_RS12990 Protein ID WP_015491236.1
Coordinates 2776195..2776485 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag CMN_RS12995 Protein ID WP_041465310.1
Coordinates 2776482..2776715 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMN_RS12980 2774144..2775742 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
CMN_RS14870 2775770..2775874 - 105 WP_080606018.1 hypothetical protein -
CMN_RS12985 2775901..2776155 + 255 Protein_2575 helix-turn-helix transcriptional regulator -
CMN_RS12990 2776195..2776485 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CMN_RS12995 2776482..2776715 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
CMN_RS13000 2776827..2777027 - 201 WP_015491238.1 hypothetical protein -
CMN_RS13005 2777043..2777270 - 228 WP_015491239.1 antitoxin VbhA family protein -
CMN_RS13010 2777353..2778426 + 1074 WP_155119035.1 LD-carboxypeptidase -
CMN_RS13015 2778603..2778962 + 360 WP_015491241.1 hypothetical protein -
CMN_RS13020 2779123..2779638 + 516 WP_041465311.1 hypothetical protein -
CMN_RS13025 2779785..2780822 + 1038 WP_015491244.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T29297 WP_015491236.1 NC_020891:c2776485-2776195 [Clavibacter nebraskensis NCPPB 2581]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T29297 NC_020891:c2776485-2776195 [Clavibacter nebraskensis NCPPB 2581]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT29297 WP_041465310.1 NC_020891:c2776715-2776482 [Clavibacter nebraskensis NCPPB 2581]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT29297 NC_020891:c2776715-2776482 [Clavibacter nebraskensis NCPPB 2581]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References