Detailed information of TA system TA029296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2894041..2895643
• Replicon: chromosome
• Accession: NC_020888
• Organism: Thalassolituus oleivorans MIL-1

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: TOL_RS13270
• Protein ID: WP_051052417.1
• Coordinates: 2894041..2894991 (-)
• Length: 317 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: M5DUR9
• Locus tag: TOL_RS13275
• Protein ID: WP_015487862.1
• Coordinates: 2895038..2895643 (-)
• Length: 202 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL_RS13260	2889572..2890054	+	483	WP_025264886.1	SsrA-binding protein SmpB	-
TOL_RS13265	2890603..2893899	-	3297	WP_015487860.1	type I restriction endonuclease subunit R	-
TOL_RS13270	2894041..2894991	-	951	WP_051052417.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
TOL_RS13275	2895038..2895643	-	606	WP_015487862.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
TOL_RS13280	2896024..2897709	-	1686	WP_015487863.1	DUF262 domain-containing HNH endonuclease family protein	-
TOL_RS13285	2897727..2898419	-	693	WP_015487864.1	hypothetical protein	-
TOL_RS13290	2898416..2899795	-	1380	WP_015487865.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-272)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-55)

Sequences

Toxin

Protein

• Length: 317 a.a.
• Molecular weight: 35696.89 Da
• Isoelectric Point: 5.6737

>T29296 WP_051052417.1 NC_020888:c2894991-2894041 [Thalassolituus oleivorans MIL-1]
MVTNKAASIRQKLLNLSRERGESFDFVLQQYAIQRLLYRLSVSEYHDQFLLKGAMLFSVWTGDLHRPTKDIDLLGFGSND
TDVLVNDFKVICAIEADDGLIFDIESIQGVRIKEDSLYQGVRVTGFAHLEKAKIGLQVDIGFGDAVTPNARLATIPSFLD
FPAPIMKIYPVYTVIAEKFHAMVALGLFNSRIKDFYDIWMIAIHGLQEGDFLLGGDALDGTALDGTLLVSAIRSTFNRRN
TVMSTEMLSVFTAEFMQDTNKHKQWNAFLNKNRIVSDQSFENIQQGLRRFLEPAYQAAALGAEYPFKWDVSEGKWK

Nucleotide

• Length: 951 bp

>T29296 NC_020888:c2894991-2894041 [Thalassolituus oleivorans MIL-1]
TTGGTGACTAATAAAGCGGCGTCGATACGCCAAAAACTGCTGAACTTATCGCGCGAACGCGGTGAGTCATTTGATTTCGT
GTTACAGCAGTATGCGATTCAGCGGCTATTGTATCGTTTGAGTGTGTCGGAGTATCACGATCAGTTTCTTTTGAAAGGGG
CGATGTTATTTTCGGTTTGGACGGGAGATCTGCATCGTCCTACTAAAGATATTGATTTGTTGGGTTTTGGATCAAACGAT
ACTGATGTACTAGTAAATGATTTCAAAGTCATTTGTGCTATAGAAGCCGATGATGGTTTGATCTTTGATATTGAATCCAT
TCAAGGTGTTCGAATTAAGGAAGACTCACTTTATCAAGGTGTGAGAGTCACAGGGTTTGCTCATTTAGAAAAAGCGAAAA
TAGGTTTGCAAGTTGATATTGGTTTTGGCGATGCTGTCACGCCTAATGCTAGGCTGGCAACTATTCCGTCATTTTTAGAT
TTTCCTGCTCCTATAATGAAGATCTATCCGGTTTATACGGTGATCGCTGAGAAGTTTCACGCGATGGTGGCGCTGGGTCT
GTTCAATAGTCGTATTAAAGATTTTTATGATATTTGGATGATTGCTATTCATGGCTTACAGGAAGGTGATTTTTTACTCG
GCGGGGATGCTCTCGATGGGACTGCTCTTGATGGTACTCTACTAGTCTCTGCCATTAGATCGACGTTCAATCGGCGGAAT
ACGGTGATGAGCACAGAGATGCTGTCTGTTTTTACGGCTGAATTTATGCAAGATACGAACAAACATAAACAGTGGAATGC
CTTCTTGAATAAAAATCGAATCGTCAGTGATCAGTCTTTTGAAAATATACAGCAAGGGTTACGACGTTTTTTAGAGCCCG
CTTATCAAGCAGCCGCCCTAGGCGCTGAATATCCTTTCAAGTGGGATGTCAGTGAAGGGAAATGGAAATAA

Antitoxin

Protein

• Length: 202 a.a.
• Molecular weight: 22783.70 Da
• Isoelectric Point: 10.2027

>AT29296 WP_015487862.1 NC_020888:c2895643-2895038 [Thalassolituus oleivorans MIL-1]
MSKTSKRQQVINLAIQRGVIRPRDLLAHGLPKDYLNILAREGVLFKLGRGLYQWPDREASRHKSLIEVAKLAPNGVVALL
SALSFHGLGTQNPFEVWLAIDRKSRRPRIHYPPVRFVTMSHEGLVEGVEQHEIDGVMVSVFCPAKTVADCFKYRNKIGLD
VALEALKDGWRAQMFTMDDLLRYAKVCRVAKVMQPYMESLG

Nucleotide

• Length: 606 bp

>AT29296 NC_020888:c2895643-2895038 [Thalassolituus oleivorans MIL-1]
ATGTCTAAAACCTCAAAACGCCAACAAGTGATCAATCTCGCCATACAGCGAGGGGTTATACGTCCGCGCGATTTGCTGGC
TCACGGCTTGCCCAAAGACTACTTAAACATTTTAGCGCGTGAAGGCGTGTTGTTTAAGCTGGGCCGTGGTTTGTATCAAT
GGCCAGATCGTGAAGCCAGCCGCCACAAGTCTTTGATTGAGGTCGCTAAACTGGCACCTAATGGTGTGGTGGCGCTGCTG
TCTGCTTTGAGTTTTCATGGCTTGGGTACACAAAATCCTTTTGAAGTTTGGTTGGCAATTGACCGCAAGTCGCGCCGTCC
TCGTATTCATTATCCGCCGGTGCGCTTTGTCACTATGTCTCATGAAGGTTTGGTTGAAGGTGTTGAGCAGCATGAAATCG
ATGGCGTAATGGTTTCGGTTTTTTGTCCGGCGAAAACCGTAGCCGATTGTTTTAAGTACCGTAATAAGATTGGGTTAGAC
GTCGCCCTAGAAGCGCTAAAGGATGGTTGGCGTGCACAAATGTTCACGATGGATGATCTGCTTAGGTACGCCAAGGTGTG
TCGTGTGGCGAAGGTTATGCAACCTTATATGGAGTCTTTGGGTTGA

Structures

Antitoxin

• AlphaFold DB: M5DUR9