Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4372920..4373496 Replicon chromosome
Accession NZ_LS992192
Organism Escherichia coli isolate Escherichia coli str. TO217

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag ECTO217_RS22150 Protein ID WP_001295743.1
Coordinates 4373209..4373496 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag ECTO217_RS22145 Protein ID WP_000063148.1
Coordinates 4372920..4373222 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECTO217_RS22130 4369516..4371666 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
ECTO217_RS22135 4371716..4371919 + 204 WP_000467859.1 YbdD/YjiX family protein -
ECTO217_RS22140 4371930..4372886 + 957 WP_001295745.1 GTPase -
ECTO217_RS22145 4372920..4373222 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
ECTO217_RS22150 4373209..4373496 - 288 WP_001295743.1 BrnT family toxin Toxin
ECTO217_RS22155 4373693..4374859 + 1167 WP_000800831.1 restriction endonuclease -
ECTO217_RS22160 4374923..4376542 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
ECTO217_RS22165 4376532..4377836 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T292954 WP_001295743.1 NZ_LS992192:c4373496-4373209 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT292954 WP_000063148.1 NZ_LS992192:c4373222-4372920 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References