Detailed information of TA system TA029295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2164924..2166614
• Replicon: chromosome
• Accession: NC_020888
• Organism: Thalassolituus oleivorans MIL-1

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: W8FWZ9
• Locus tag: TOL_RS09850
• Protein ID: WP_015487173.1
• Coordinates: 2164924..2166243 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: TOL_RS09855
• Protein ID: WP_015487174.1
• Coordinates: 2166240..2166614 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL_RS09835	2160908..2162011	+	1104	WP_015487169.1	acyl-CoA desaturase	-
TOL_RS19160	2162047..2162148	+	102	WP_197537887.1	DUF2789 family protein	-
TOL_RS19165	2162115..2162210	+	96	WP_155990895.1	DUF2789 family protein	-
TOL_RS09840	2162405..2164348	+	1944	WP_015487171.1	EAL domain-containing protein	-
TOL_RS18360	2164389..2164853	+	465	WP_015487172.1	hypothetical protein	-
TOL_RS09850	2164924..2166243	-	1320	WP_015487173.1	type II toxin-antitoxin system HipA family toxin	Toxin
TOL_RS09855	2166240..2166614	-	375	WP_015487174.1	helix-turn-helix domain-containing protein	Antitoxin
TOL_RS09860	2166783..2167331	+	549	WP_015487175.1	YaeQ family protein	-
TOL_RS09865	2167341..2168702	+	1362	WP_015487176.1	DEAD/DEAH box helicase	-
TOL_RS09870	2168767..2169609	-	843	WP_015487177.1	zinc-dependent peptidase	-
TOL_RS09875	2169643..2170065	-	423	WP_051052472.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (175-415)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49117.93 Da
• Isoelectric Point: 6.7689

>T29295 WP_015487173.1 NC_020888:c2166243-2164924 [Thalassolituus oleivorans MIL-1]
VISVAEVRLWGITIGAVQWDAQRQLGFFEYAKPFLRSGIQLSPLHMPLGAGVFSFPALNKESYKGLPGMLADSLPDKFGN
LLIDQWLVSQNREPGSFNPVERLLYMGIRGMGALEYLPETPRTRIPDQQLEMTKLVDLANRALAQKDSLAAKFGYSANDS
MADTQALQQIISVGTSAGGARAKAVIAWNEATHEVRSGQLDCPEGFEHYLLKFDGVQGNRDKELNDPQGFTRIEYAYSQM
AKAAGIDMMPCQLLEENGRAHFLTKRFDRIGGKDKLHMQTLCGVAHFDFNMAGAYSYEQAFRVMRQMGLQDLHLALEQQF
RRMVFNVMARNQDDHTKNIAFLMNKRGEWRLSPAYDVTYSYNPSGEWTSSHQMSVNGKRDGFVLDDLLACGAMADVSERR
VRAIVGEVAEVLKTWSQYADDAGIEASWATKIGASFRKI

Nucleotide

• Length: 1320 bp

>T29295 NC_020888:c2166243-2164924 [Thalassolituus oleivorans MIL-1]
GTGATTTCTGTGGCAGAAGTGCGTCTCTGGGGTATAACCATTGGTGCTGTGCAATGGGATGCGCAGCGTCAGCTTGGTTT
CTTTGAATACGCTAAGCCTTTTTTACGCTCGGGCATTCAGCTTTCGCCATTGCATATGCCGCTGGGTGCCGGCGTGTTTT
CATTTCCTGCTTTGAATAAAGAATCGTACAAGGGTTTGCCAGGTATGTTGGCGGACTCTTTGCCTGATAAATTCGGTAAT
TTACTGATAGATCAGTGGTTAGTGAGTCAGAATCGCGAACCGGGCAGTTTTAACCCGGTGGAACGTCTTTTATATATGGG
CATACGTGGTATGGGCGCGCTTGAGTATTTACCAGAAACTCCACGCACTCGCATTCCTGATCAGCAATTAGAAATGACTA
AACTGGTGGATTTGGCTAATCGAGCCCTAGCTCAAAAGGATAGCTTGGCTGCTAAGTTTGGTTATTCTGCCAATGACTCT
ATGGCCGATACTCAAGCCTTGCAGCAGATTATTTCTGTGGGTACGTCGGCTGGTGGTGCTCGCGCTAAAGCCGTGATTGC
ATGGAATGAGGCAACTCATGAAGTTCGCTCTGGGCAATTGGATTGTCCTGAGGGTTTTGAGCATTATTTGTTGAAGTTCG
ATGGTGTGCAGGGTAACCGTGATAAAGAGTTGAATGATCCACAAGGGTTTACTCGTATCGAATATGCCTACTCGCAAATG
GCTAAAGCCGCTGGGATCGACATGATGCCGTGTCAGTTGTTAGAAGAAAACGGGCGCGCGCACTTTCTAACAAAACGCTT
TGATCGAATTGGCGGCAAAGATAAATTGCACATGCAAACGCTCTGTGGTGTTGCGCACTTCGATTTTAATATGGCGGGTG
CGTATTCTTATGAGCAAGCGTTTCGGGTGATGCGCCAAATGGGCTTGCAGGATTTGCATCTAGCGCTTGAGCAGCAATTT
CGTCGTATGGTGTTTAACGTTATGGCACGTAATCAGGACGACCATACGAAAAATATCGCCTTCTTAATGAATAAGCGCGG
TGAATGGCGCTTGTCTCCCGCTTATGATGTGACCTATTCGTATAATCCGAGCGGCGAGTGGACATCGAGTCATCAAATGA
GTGTTAACGGCAAACGCGATGGATTTGTGCTGGATGATCTGCTTGCTTGTGGCGCGATGGCGGATGTCAGTGAGCGCCGT
GTTAGAGCGATAGTGGGTGAAGTGGCGGAGGTATTAAAAACTTGGTCTCAATATGCTGACGATGCGGGTATTGAGGCAAG
TTGGGCGACTAAGATTGGCGCCAGCTTCCGTAAAATATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13322.93 Da
• Isoelectric Point: 4.6017

>AT29295 WP_015487174.1 NC_020888:c2166614-2166240 [Thalassolituus oleivorans MIL-1]
MEYSSVSNLVVLEQLGERFRSARLSRNLTQAQLAEASGVSLSTLRRLESGADNVSLMNIIALMRALGVVDQLELLLPPQP
PSPMASLQNSGAGPQVDNGSERQRASGSRQVAEDKGTWTWGDDA

Nucleotide

• Length: 375 bp

>AT29295 NC_020888:c2166614-2166240 [Thalassolituus oleivorans MIL-1]
ATGGAATACTCATCAGTTAGTAATTTAGTCGTGCTAGAGCAGTTGGGTGAGCGTTTTCGCAGTGCTCGTTTAAGCCGGAA
CTTAACTCAAGCGCAGTTGGCTGAGGCATCGGGCGTCAGCTTGTCGACGCTAAGGCGTTTGGAGAGCGGGGCGGATAACG
TCAGCTTAATGAATATTATTGCCTTAATGCGCGCCCTGGGTGTGGTCGATCAACTCGAATTGTTACTACCGCCTCAGCCT
CCTAGTCCTATGGCGAGCTTGCAGAATAGCGGTGCAGGGCCGCAAGTCGATAACGGCAGCGAGCGGCAGCGTGCATCTGG
CAGCCGTCAGGTAGCCGAAGATAAGGGCACTTGGACATGGGGCGATGACGCGTGA

Structures

Toxin

• AlphaFold DB: W8FWZ9