Detailed information of TA system TA029294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 1310196..1310706
• Replicon: chromosome
• Accession: NC_020888
• Organism: Thalassolituus oleivorans MIL-1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: W8GA76
• Locus tag: TOL_RS05920
• Protein ID: WP_015486389.1
• Coordinates: 1310443..1310706 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W8G613
• Locus tag: TOL_RS05915
• Protein ID: WP_015486388.1
• Coordinates: 1310196..1310450 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL_RS05880	1306049..1306825	+	777	WP_015486381.1	nucleotidyltransferase domain-containing protein	-
TOL_RS05885	1306947..1307387	+	441	WP_015486382.1	hypothetical protein	-
TOL_RS05890	1307498..1307974	+	477	WP_041588416.1	hypothetical protein	-
TOL_RS05895	1308091..1308579	+	489	WP_015486384.1	hypothetical protein	-
TOL_RS05900	1308576..1308881	+	306	WP_015486385.1	hypothetical protein	-
TOL_RS05905	1308984..1309391	+	408	WP_015486386.1	GNAT family N-acetyltransferase	-
TOL_RS05910	1309486..1310100	+	615	WP_015486387.1	transglutaminase family protein	-
TOL_RS05915	1310196..1310450	+	255	WP_015486388.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
TOL_RS05920	1310443..1310706	+	264	WP_015486389.1	Txe/YoeB family addiction module toxin	Toxin
TOL_RS18915	1310803..1311162	+	360	WP_015486390.1	IS3 family transposase	-
TOL_RS05930	1311099..1311878	+	780	WP_144055336.1	IS3 family transposase	-
TOL_RS05935	1312018..1312773	-	756	WP_015486392.1	TSUP family transporter	-
TOL_RS05940	1312830..1315352	-	2523	WP_015486393.1	ATP-dependent helicase HrpB	-
TOL_RS05945	1315428..1315610	+	183	WP_015486394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1310803..1311162	359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10365.75 Da
• Isoelectric Point: 9.0098

>T29294 WP_015486389.1 NC_020888:1310443-1310706 [Thalassolituus oleivorans MIL-1]
MSSRLLCWTDEAWNDYVYWQGQDKKTLKRINKLISDTKRSPFEGIGKPESLKENLSGFWSRRIDDTNRLVYAVDDKTLTI
ISCRYHY

Nucleotide

• Length: 264 bp

>T29294 NC_020888:1310443-1310706 [Thalassolituus oleivorans MIL-1]
ATGAGTAGTAGGCTTCTTTGTTGGACCGATGAAGCTTGGAATGATTACGTTTACTGGCAAGGTCAGGACAAAAAGACATT
AAAGCGTATAAACAAATTAATCAGTGACACTAAGCGTTCACCATTTGAGGGTATTGGCAAGCCTGAGTCTCTTAAAGAAA
ATTTGTCAGGTTTCTGGTCACGCCGAATTGATGACACCAATCGTTTAGTTTATGCCGTTGATGATAAAACGCTAACCATA
ATATCGTGCAGGTATCATTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9373.51 Da
• Isoelectric Point: 5.0481

>AT29294 WP_015486388.1 NC_020888:1310196-1310450 [Thalassolituus oleivorans MIL-1]
MRVVSFTEARNGLKAVLDSVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLKSPANAEHLNKSIAQYKSGKTTQRD
IVDE

Nucleotide

• Length: 255 bp

>AT29294 NC_020888:1310196-1310450 [Thalassolituus oleivorans MIL-1]
ATGAGAGTTGTATCTTTTACTGAAGCAAGAAATGGATTAAAAGCTGTTTTAGACTCTGTCGTTAATGATGCAGACACCAC
GGTTATAACGCGTCGAGATTCAGAAGATGCGGTTGTAATGTCATTAGATTATTACAATAGTTTGATGGAAACAGTTCATT
TATTAAAGTCTCCTGCAAATGCAGAACATTTAAACAAATCAATAGCTCAATATAAAAGTGGTAAAACTACGCAAAGAGAT
ATAGTCGATGAGTAG

Structures

Toxin

• AlphaFold DB: W8GA76

Antitoxin

• AlphaFold DB: W8G613